CORE--GENE ANALYSIS

这项拨款提案中的所有三个研究项目都有共同的目标 解开ETS家族的发育和致癌功能 转录因子。虽然不同的系统在 在单个项目中，许多实验方法对所有人都是通用的。一 这项赠款提案的基本理念是， 分子与结构生物学(CMSB)可以提供共享设施和 向拟议的研究项目提供技术专长；以及 整合我们能够负担得起的资源，高效和 为这项赠款提案的目标提供经济支持。 基因表达模式的检测和可能的特征 下游基因是理解ETS基因功能的中心。在……里面 根据这一理解，有两个领域的实验需求 被确定为必不可少的，并且最能满足核心的需求 设施。它们是(A)DNA序列分析和(B)原位检测 基因表达模式。 该核心目标是：1)为以下方面提供综合资源 DNA序列分析。2)提供标准化的方法和共享 用于检测基因表达模式的设备。

All three research projects in this grant proposal share the common goal in unraveling the developmental and oncogenic functions of the Ets family of transcription factors. Although different systems are used in the individual projects, many experimental approaches are common to all. One underlying philosophy of this grant proposal is that the Center for Molecular and Structural Biology (CMSB) can provide share facility and technical expertise to the proposed research projects; and by consolidating the resources we will be able to afford efficient and economical support for the objectives of this grant proposal. Examination of gene expression patterns and characterization of possible down-stream genes are central to understanding the ets gene functions. In accordance with this understanding, two areas of experimental needs have been identified to be essential and can be best satisfied by core facilities. These are (A) DNA sequence analysis and (B) in situ detection of gene expression patterns. The objective of this core are: 1) To provide consolidated resource for DNA sequence analysis. 2) To provide standardized methodology and shared facilities for examining gene expression patterns.