MOLECULAR CHARACTERIZATION OF H2 RECEPTOR DUAL SIGNALING

H2 受体双信号传导的分子表征

• 批准号: 6380848
• 负责人: JOHN DELVALLE
• 金额: $ 33.07万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-30 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6380848
• 关键词: G protein; adenylate cyclase; biological signal transduction; cell differentiation; cell growth regulation; chimeric proteins; gastric mucosa; histamine receptor; phospholipase C; receptor coupling; site directed mutagenesis; tissue /cell culture; western blottings

DESCRIPTION (Adapted from applicant's abstract): This is a proposal to continue studies on signaling via histamine H2 receptors in gastric parietal cells. The Principal Investigator (PI) and his colleagues have characterized the molecular structure and function of the cloned H2 receptor and determined that it activates adenylate cyclase and phospholipase C (PLC) in a GTP-dependent, cholera toxin-sensitive manner. The mechanisms whereby the receptor couples to two effector systems remain to be determined. Furthermore, the PI has recently found that the receptor mediates cell proliferation and transcriptional activation of the early response gene, c-fos. In the current proposal the following will be examined. (1) Chimeric receptor constructs and site directed mutagenesis will be used to elucidate the structural components of the receptor linked to activation of adenylate cyclase and phospholipase C; (2) the G proteins involved in H2 receptor signaling in primary and transformed cells will be characterized using selective antibodies, immunoblotting, GTP-labeling and molecular techniques; and (3) the biological significance of dual signaling will be explored. H2 receptor/G protein targeted oligopeptides, antisera and antisense probes will be used to determine the relative importance of the two signaling pathways in cell proliferation and parietal cell secretory activity.

描述（改编自申请人摘要）：这是一份关于

项目成果

Regulation of c-Jun NH2-terminal kinases in isolated canine gastric parietal cells.

离体犬胃壁细胞中 c-Jun NH2 末端激酶的调节。

• DOI: 10.1152/ajpgi.1998.275.4.g740
• 发表时间: 1998
• 期刊: The American journal of physiology
• 作者: Nagahara,A;Wang,L;DelValle,J;Todisco,A
• 通讯作者: Todisco,A

Lansoprazole and ranitidine affect the accuracy of the 14C-urea breath test by a pH-dependent mechanism.

兰索拉唑和雷尼替丁通过 pH 依赖性机制影响 14C-尿素呼气试验的准确性。

• 发表时间: 1997
• 期刊: The American journal of gastroenterology.
• 作者: Chey,WD;Woods,M;Scheiman,JM;Nostrant,TT;DelValle,J
• 通讯作者: DelValle,J

Bismuth subsalicylate instead of metronidazole with lansoprazole and clarithromycin for Helicobacter pylori infection: a randomized trial.

次水杨酸铋代替甲硝唑与兰索拉唑和克拉霉素治疗幽门螺杆菌感染：一项随机试验。

• 发表时间: 1997
• 期刊: The American journal of gastroenterology.
• 作者: Chey,WD;Fisher,L;Elta,GH;Barnett,JL;Nostrant,T;DelValle,J;Hasler,WL;Scheiman,JM
• 通讯作者: Scheiman,JM