Biomaterials for Orbit Reconstruction

用于轨道重建的生物材料

• 批准号: 6338028
• 负责人: RAJENDRA S BHATNAGAR
• 金额: $ 10万
• 依托单位: BIOMIMESYS, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-30 至 2002-03-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6338028
• 关键词: alkaline phosphatase; biomaterial development /preparation; biomimetics; bone morphogenetic proteins; bone regeneration; cell adhesion; cell differentiation; cell migration; cell proliferation; collagen; electron microscopy; gene expression; immunocytochemistry; northern blottings; osteocalcin; osteocytes; osteonectin; polymerase chain reaction; tissue /cell culture; tissue engineering; tissue support frame; transforming growth factors

DESCRIPTION (Verbatim from Applicant): We will develop biomimetic materials for repair and orthoplasty of the orbit and surrounding bony structures. These scaffolds will incorporate a cell binding peptide related to collagen which will allow them to serve as collagen-like anchorages for cells. These Collagen Surrogate Matrices (CSM) will facilitate haptotactic migration of cells from host tissue, promote 3-D colony formation, and they will provide a permissive environment for cell differentiation and tissue remodeling. In Phase I studies, we will (1) examine quantitatively the adhesion, migration, and colonization of CSM by osteoblast-like cell lines, (2) characterize the morphology of cellular colonies using histological and electron microscopic procedures, (3) determine the effect of various growth factors on the attachment, proliferation, and differentiation of cells on synthetic scaffolds, and (4) examine the expression of bone-specific molecules (type I collagen, alkaline phosphatase, osteonectin, osteocalcin), and growth factors (TGF-B1, BMPs 2, 4, 6 and 7) using PCR. In Phase II we will examine the performance of these matrices as grafts in animal models as space fillers in tissues. CSM will be also useful for tissue augmentation and tissue engineering. PROPOSED COMMERCIAL APPLICATIONS: There is a major need for biomaterials for repair of the bony orbit and surrounding cranio-facial skeletal structures. Such materials can also be useful for repair of bone elsewhere. The overall market value of biomaterials for orthopedic grafts and implants is estimated to be in billions of dollars.

描述（申请人逐字）：我们将开发仿生材料 眼眶和周围骨结构的修复和矫形术。这些 支架将包含与胶原蛋白相关的细胞结合肽， 将使它们能够充当细胞的胶原蛋白样锚定物。这些胶原蛋白 替代矩阵（CSM）将促进细胞的趋触迁移 宿主组织，促进 3-D 集落形成，并且它们将提供许可 细胞分化和组织重塑的环境。在第一阶段研究中， 我们将 (1) 定量地检查以下物质的粘附、迁移和定植 CSM通过成骨细胞样细胞系，（2）表征细胞的形态 使用组织学和电子显微镜程序进行集落，(3) 确定 各种生长因子对附着、增殖和生长的影响 细胞在合成支架上的分化，以及（4）检查表达 骨特异性分子（I 型胶原蛋白、碱性磷酸酶、骨连接蛋白、 使用 PCR 检测骨钙素）和生长因子（TGF-B1、BMP 2、4、6 和 7）。在 第二阶段，我们将检查这些基质作为动物移植物的性能 模型作为组织中的空间填充物。 CSM 对组织也很有用 增强和组织工程。 拟议的商业应用： 修复骨性眼眶和周围颅面骨骼结构非常需要生物材料。此类材料还可用于修复其他部位的骨骼。用于骨科移植物和植入物的生物材料的总体市场价值估计为数十亿美元。