Long-range control of tissue-specific expression in Arabidopsis

基因表达调控对所有活着的生物体都是至关重要的。基因组结构的功能分析揭示了位于编码序列远端的调控元件的存在。为了了解这些调控元件的组合活性如何影响基因活性的时效性和特殊性，定义基因调控的构成要素是至关重要的。理解这种远程调控是如何发生的，并揭示这一过程中涉及的机制是生物学中两个基本的悬而未决的问题。与动物的情况相反，我们仍然对长距离调控序列在植物基因表达调控中所起的作用一无所知。这项研究的主要目的是识别和表征与实验模式植物拟南芥基因表达的时空调控有关的序列，以及它们如何受到环境波动的影响。我们将通过调控元件的有针对性的操作和全基因组分析来阐明特定的基因功能。这些知识将对设计出更好地适应环境压力的作物物种至关重要。

Gene expression regulation is critical for all living organisms. Functional analyses of genomic architecture have revealed the existence of regulatory elements residing distal to coding sequences. To understand how the combined activity of these regulatory elements affects the temporal and special regulation of gene activity, it is essential to define the constitutive elements of gene regulation. Understanding how this long-distance regulation occurs and uncovering the mechanisms involved in this process are two fundamental unresolved questions in biology. Contrary to the situation in animals, we remain ignorant of the role played by long-distance regulatory sequences in gene expression regulation in plants. The main objective of this study is to identify and characterise the sequences that are involved in the spatial and temporal regulation of gene expression in the experimental model plant, Arabidopsis thaliana, and how they are influenced by environmental fluctuations. We will elucidate specific gene function through the targeted manipulation of regulatory elements and genome-wide analyses. This knowledge will be critical to engineer crop species that are better adapted to environmental stress.