Long-range control of tissue-specific expression in Arabidopsis

拟南芥组织特异性表达的远程控制

• 批准号: 1643084
• 金额: --
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1643084
• 关键词: Long; range; control; tissue; specific

Gene expression regulation is critical for all living organisms. Functional analyses of genomic architecture have revealed the existence of regulatory elements residing distal to coding sequences. To understand how the combined activity of these regulatory elements affects the temporal and special regulation of gene activity, it is essential to define the constitutive elements of gene regulation. Understanding how this long-distance regulation occurs and uncovering the mechanisms involved in this process are two fundamental unresolved questions in biology. Contrary to the situation in animals, we remain ignorant of the role played by long-distance regulatory sequences in gene expression regulation in plants. The main objective of this study is to identify and characterise the sequences that are involved in the spatial and temporal regulation of gene expression in the experimental model plant, Arabidopsis thaliana, and how they are influenced by environmental fluctuations. We will elucidate specific gene function through the targeted manipulation of regulatory elements and genome-wide analyses. This knowledge will be critical to engineer crop species that are better adapted to environmental stress.

基因表达调控对所有生物体都至关重要。基因组结构的功能分析揭示了存在的调控元件的远端编码序列。为了理解这些调控元件的组合活性如何影响基因活性的时间和特殊调控，定义基因调控的组成元件是至关重要的。了解这种长距离调节是如何发生的，并揭示这一过程中涉及的机制是生物学中两个尚未解决的基本问题。与动物的情况相反，我们仍然不知道长距离调控序列在植物基因表达调控中所起的作用。本研究的主要目的是鉴定和分析实验模式植物拟南芥中参与基因表达时空调控的序列，以及它们如何受到环境波动的影响。我们将通过调控元件的靶向操作和全基因组分析来阐明特定的基因功能。这些知识对于设计更好地适应环境压力的作物物种至关重要。