NITRIC OXIDE ACTION IN PRIMATE MYOMETRIUM

描述(改编自《调查者摘要》)：这两次 修订后的新R29申请，迈克尔·E·布拉德利博士申请350,000美元 研究“一氧化氮分子基础”的直接成本超过5年 在灵长类肌层中的作用。这个项目的长期目标是 了解导致子宫收缩开始的信号 与劳动有关的。这项提案的具体目的是确定是否 人类或猴子子宫的组织产生一氧化氮，以及 一氧化氮可以松弛人或猴子的子宫肌细胞。该提案是 分为两个具体目标。在具体目标1中，调查员将 确定外源性NO在非妊娠、近月妊娠、 以及劳累的子宫组织和细胞。在这一具体目标中， 研究人员将确定一氧化氮对子宫肌层组织张力的影响 并测定NO对钙离子浓度、cAMP浓度和 CGMP。在具体目标2中，调查员将描述、定位和 探讨灵长类动物子宫中一氧化氮合酶亚型的调控。 在这一特定目的中要探索的技术包括蛋白质印迹分析 完整组织和培养细胞中一氧化氮合酶mRNA的检测 逆转录-聚合酶链式反应。

DESCRIPTION (Adapted from the Investigator's Abstract): In this twice revised new R29 application, Michael E. Bradley, Ph.D. requests $350,000 in direct costs over 5 years to study the "molecular basis of nitric oxide action in primate myometrium." The long-term goal of this project is to understand the signals responsible for commencement of uterine contractions associated with labor. The specific aim of this proposal is to determine if the tissues of the human or monkey uterus produce nitric oxide and whether nitric oxide can relax human or monkey uterine myocytes. The proposal is divided into 2 specific aims. In specific aim 1 the investigator will determine the effects of exogenous NO in non-pregnant, near term pregnant, and laboring uterine tissues and cells. In this specific aim the investigator will determine the effects of NO on myometrial tissue tension and determine the effects on NO on the concentration of calcium, cAMP and cGMP. In specific aim 2 the investigator will characterize, localize and investigate the regulation of NOS isoforms in the primate uterus. Techniques to be explored in this specific aim include Western blot analysis of intact tissues and cultured cells and determination of NOS mRNA expression using RT-PCR.