Defining the role of Death Associated Protein Kinase 1 in cell fate using genomic and proteomic analysis

使用基因组和蛋白质组分析定义死亡相关蛋白激酶 1 在细胞命运中的作用

• 批准号: 1644051
• 金额: --
• 依托单位: University of Reading
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1644051
• 关键词: Defining; role; Death; Associated; Protein

Project Overview: Death associated protein kinase 1 (DAPK1) is key regulator of cell fate in human cells, acting as a control point for autophagic cell death, and has been implicated in cancer and neurodegneration. Despite two decades of research, the molecular pathways coordinated by DAPK1 remain poorly defined.The aim of this project is to use new advances in genomic and proteomic technology, coupled with novel insights into the enzymatic function of DAPK1, to dissect the pathways downstream of this protein. This will, in turn, help define the cellular role of this important protein and aid in the development of new drugs to modulate DAPK1.This four year studentship, funded by an Industrial CASE award from the BBSRC, will be based partly at the University of Reading School of Pharmacy and partly at BC platforms, a leader in the field of bioinformatics. The student will use cellular and biochemical methods to characterise DAPK1, leading on to genomic and proteomic analysis of the cellular impact of modulating the enzymes activity. Bioinformatic investigation of the latter will be carried out with BC platforms, providing industry experience. During the course of the PhD, the student will have the opportunity to register for a Certificate in Business Administration at Henley Business School, part of the University of Reading.

项目概述：死亡相关蛋白激酶1（DAPK1）是人类细胞命运的关键调节因子，作为自噬细胞死亡的控制点，并与癌症和神经变性有关。本项目的目的是利用基因组学和蛋白质组学技术的新进展，结合对DAPK 1酶功能的新认识，来剖析这种蛋白质下游的通路。这将反过来帮助定义这种重要蛋白质的细胞作用，并帮助开发新的药物来调节DAPK1。这四年的学生，由BBSRC的工业案例奖资助，将部分基于阅读药学院的大学，部分基于生物信息学领域的领导者BC平台。学生将使用细胞和生物化学方法来研究DAPK1，从而对调节酶活性的细胞影响进行基因组和蛋白质组学分析。后者的生物信息学调查将通过BC平台进行，提供行业经验。在博士课程期间，学生将有机会在阅读大学的亨利商学院注册工商管理证书。

项目成果

Measuring Lactase Enzymatic Activity in the Teaching Lab.

在教学实验室中测量乳糖酶活性。

• DOI: 10.3791/54377
• 发表时间: 2018-08-06
• 期刊: Journal of visualized experiments : JoVE
• 作者: Leksmono CS;Manzoni C;Tomkins JE;Lucchesi W;Cottrell G;Lewis PA
• 通讯作者: Lewis PA

PINOT: an intuitive resource for integrating protein-protein interactions.

PINOT：整合蛋白质-蛋白质相互作用的直观资源。

• DOI: 10.1186/s12964-020-00554-5
• 发表时间: 2020
• 期刊: CCS
• 作者: Tomkins JE

Stratification of candidate genes for Parkinson's disease using weighted protein-protein interaction network analysis.

• DOI: 10.1186/s12864-018-4804-9
• 发表时间: 2018-06-13
• 期刊: BMC genomics
• 影响因子: 4.4
• 作者: Ferrari R;Kia DA;Tomkins JE;Hardy J;Wood NW;Lovering RC;Lewis PA;Manzoni C
• 通讯作者: Manzoni C

Additional rare variant analysis in Parkinson's disease cases with and without known pathogenic mutations: evidence for oligogenic inheritance.

• DOI: 10.1093/hmg/ddw348
• 发表时间: 2016-12-15
• 期刊: Human molecular genetics
• 影响因子: 3.5
• 作者: Lubbe SJ;Escott-Price V;Gibbs JR;Nalls MA;Bras J;Price TR;Nicolas A;Jansen IE;Mok KY;Pittman AM;Tomkins JE;Lewis PA;Noyce AJ;Lesage S;Sharma M;Schiff ER;Levine AP;Brice A;Gasser T;Hardy J;Heutink P;Wood NW;Singleton AB;Williams NM;Morris HR;for International Parkinson’s Disease Genomics Consortium
• 通讯作者: for International Parkinson’s Disease Genomics Consortium

Comparative Protein Interaction Network Analysis Identifies Shared and Distinct Functions for the Human ROCO Proteins.

• DOI: 10.1002/pmic.201700444
• 发表时间: 2018-05
• 期刊: Proteomics
• 影响因子: 3.4
• 作者: Tomkins JE;Dihanich S;Beilina A;Ferrari R;Ilacqua N;Cookson MR;Lewis PA;Manzoni C
• 通讯作者: Manzoni C