Role of TrkB and TrkC in Cocaine-Induced Sensitization
TrkB 和 TrkC 在可卡因诱导致敏中的作用
基本信息
- 批准号:6338413
- 负责人:
- 金额:$ 3.67万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2001
- 资助国家:美国
- 起止时间:2001-07-01 至
- 项目状态:未结题
- 来源:
- 关键词:behavior test behavioral /social science research tag behavioral habituation /sensitization brain derived neurotrophic factor cocaine craving dopamine enzyme activity growth factor receptors immunocytochemistry laboratory rat microinjections mitogen activated protein kinase neural plasticity receptor expression statistics /biometry tegmentum transfection /expression vector tyrosine 3 monooxygenase western blottings
项目摘要
There are several important reasons for studying the cellular and molecular
mechanisms underlying the behavioral effects of repeated psychostimulant
administration. First, abuse of psychostimulants such as cocaine is a major
medical and social problem. Studies suggest that the neurophysiological
adaptations associated with repeated psychostimulant administration contribute
to drug craving. Second, the cellular changes underlying repeated
psychostimulant administration are a form of neuronal plasticity. Studying the
psychostimulant-induced neuronal adaptations will shed more light on the
mechanisms of neuronal plasticity in general. Third, studying the molecular
adaptations associated with psychostimulant exposure will help us to understand
the neural mechanisms that lead to subjective experiences such as pleasure and
pain, one of the most fascinating problems in the field of neuroscience.
Growing evidence indicates that the neurotrophin family of growth factors plays
an important role in the effects of psychostimulants. BDNF and NT-3
microinjections into the VTA have been shown to enhance the behavioral
activating effects of cocaine. The goal of the research outlined in this
proposal is to expand on these findings by studying the role of BDNF and NT-3
receptors, trkB and trkC respectively, in cocaine-induced behavioral
sensitization. Specifically, this will be accomplished in the following two
stages. First, rats will be injected with acute and repeated systemic
injections of cocaine and levels of trkB and trkC protein in the ventral
tegmental area (VTA) will be measured. Second, trkB and trkC receptors will be
up-regulated and down-regulated in the VTA using sense and antisense
recombinant adenovirus vectors. Following virus treatment, rats will be given
acute and repeated systemic cocaine injections. Subsequent behavioral activity
will be measured and the VTA will be excised to test for changes in tyrosine
hydroxylase activity as a measure of cocaine-induced neuronal plasticity.
研究细胞和分子有几个重要原因
重复精神兴奋剂行为影响的潜在机制
行政。首先,滥用可卡因等精神兴奋剂是一个主要问题。
医疗和社会问题。研究表明,神经生理学
与重复精神兴奋剂给药相关的适应有助于
对毒品的渴望。其次,重复的细胞变化
精神兴奋剂给药是神经元可塑性的一种形式。正在学习
精神兴奋剂引起的神经元适应将有助于揭示
神经元可塑性的一般机制。三、研究分子
与精神兴奋剂暴露相关的适应将帮助我们理解
导致主观体验的神经机制,例如快乐和
疼痛,神经科学领域最令人着迷的问题之一。
越来越多的证据表明,生长因子的神经营养蛋白家族发挥着重要作用
在精神兴奋剂的作用中发挥重要作用。 BDNF 和 NT-3
VTA 显微注射已被证明可以增强行为
可卡因的激活作用。本研究概述的目标
建议通过研究 BDNF 和 NT-3 的作用来扩展这些发现
受体,trkB 和 trkC 分别在可卡因诱导的行为中
敏化。具体来说,将通过以下两个方面来实现
阶段。首先,对大鼠进行急性和重复的全身注射
可卡因注射和腹侧 trkB 和 trkC 蛋白水平
将测量被盖面积(VTA)。其次,trkB 和 trkC 受体将
使用正义和反义在 VTA 中上调和下调
重组腺病毒载体。病毒治疗后,将给予大鼠
急性和反复全身注射可卡因。随后的行为活动
将进行测量并切除 VTA 以测试酪氨酸的变化
羟化酶活性作为可卡因诱导的神经元可塑性的量度。
项目成果
期刊论文数量(0)
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会议论文数量(0)
专利数量(0)
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{{ truncateString('AUSAF BARI', 18)}}的其他基金
Deep Brain Stimulation of the Subgenual Cingulate Cortex for the Treatment of Medically Refractory Chronic Low Back Pain
膝下扣带皮层脑深部刺激治疗难治性慢性腰痛
- 批准号:
10174456 - 财政年份:2019
- 资助金额:
$ 3.67万 - 项目类别:
Deep Brain Stimulation of the Subgenual Cingulate Cortex for the Treatment of Medically Refractory Chronic Low Back Pain
膝下扣带皮层脑深部刺激治疗难治性慢性腰痛
- 批准号:
10221068 - 财政年份:2019
- 资助金额:
$ 3.67万 - 项目类别:
Deep Brain Stimulation of the Subgenual Cingulate Cortex for the Treatment of Medically Refractory Chronic Low Back Pain
膝下扣带皮层脑深部刺激治疗难治性慢性腰痛
- 批准号:
10725419 - 财政年份:2019
- 资助金额:
$ 3.67万 - 项目类别:
Deep Brain Stimulation of the Subgenual Cingulate Cortex for the Treatment of Medically Refractory Chronic Low Back Pain
膝下扣带皮层脑深部刺激治疗难治性慢性腰痛
- 批准号:
10020199 - 财政年份:2019
- 资助金额:
$ 3.67万 - 项目类别:
Role of TrkB and TrkC in Cocaine-Induced Sensitization
TrkB 和 TrkC 在可卡因诱导致敏中的作用
- 批准号:
6921495 - 财政年份:2002
- 资助金额:
$ 3.67万 - 项目类别:
Role of TrkB and TrkC in Cocaine-Induced Sensitization
TrkB 和 TrkC 在可卡因诱导致敏中的作用
- 批准号:
6603911 - 财政年份:2002
- 资助金额:
$ 3.67万 - 项目类别:
Role of TrkB and TrkC in Cocaine-Induced Sensitization
TrkB 和 TrkC 在可卡因诱导致敏中的作用
- 批准号:
6763135 - 财政年份:2002
- 资助金额:
$ 3.67万 - 项目类别:
Role of TrkB and TrkC in Cocaine-Induced Sensitization
TrkB 和 TrkC 在可卡因诱导致敏中的作用
- 批准号:
7113848 - 财政年份:2002
- 资助金额:
$ 3.67万 - 项目类别:
Role of TrkB and TrkC in Cocaine-Induced Sensitization
TrkB 和 TrkC 在可卡因诱导致敏中的作用
- 批准号:
6515898 - 财政年份:2002
- 资助金额:
$ 3.67万 - 项目类别: