Unravelling interactions between cereal aphids and ancestral wheat lines to elucidate mechanisms of aphid resistance

揭示谷类蚜虫和祖先小麦品系之间的相互作用，以阐明蚜虫抗性机制

• 批准号: 1647973
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1647973
• 关键词: Unravelling; interactions; between; cereal; aphids

The mini-project to be done at Rothamsted will focus on training the student in methods essential to the main project. This will include headspace collection of volatile organic compounds from wheat plants, bioassays to study aphid responses to resistant and susceptible lines of T. monococcum, and molecular techniques.1) The headspace volatiles from resistant and susceptible lines of T. monococcum will be collected using air entrainment in which the volatiles are collected on a porous polymer (Porapak Q50 mg), eluted with redistilled diethyl ether and then analysed using gas chromatography (GC) and GC mass spectrometry.2) Samples from headspace collections will be used to study behavioural responses of alate R. padi to the volatiles from resistant and susceptible T. monococcum using a four-arm olfactometer. The length of time spent in the treated arm is recorded and compared to control arms. Individual aphids are observed over a 16 minute period in each replicate.3) DNA from individual plants will be extracted using the DNeasy Plant Kit (Qiagen). The quantity and quality of DNA will be assessed by electrophoresis on a 0.8% agarose gel. The molecular weight of DNA in samples will be determined by comparing them to markers of known weights.

将在Rothamsted完成的小型项目将侧重于培训学生掌握主要项目所必需的方法。这将包括顶空收集小麦植株中的挥发性有机化合物，生物测定以研究蚜虫对T. 1）利用顶空挥发物分析技术，对烟草赤星病菌抗、感株系的顶空挥发物进行了分析。将使用空气夹带收集单球菌，其中挥发物收集在多孔聚合物（Porapak Q50 mg）上，用再蒸馏的乙醚洗脱，然后使用气相色谱（GC）和GC质谱分析。2）来自顶空收集的样品将用于研究alate R的行为反应。padi对抗性和感病T. monococcum使用四臂嗅觉仪。记录在治疗组中花费的时间长度并与对照组进行比较。在每次重复中观察个体蚜虫16分钟。3）使用DNeasy Plant Kit（Qiagen）提取来自个体植物的DNA。通过在0.8%琼脂糖凝胶上电泳评估DNA的数量和质量。将通过将样品中的DNA分子量与已知重量的标记物进行比较来确定样品中的DNA分子量。