IMPROVING SURFACE PLASMON RESONANCE TECHNOLOGY

描述：(改编自申请人的摘要)本申请 代表了一种跨学科的努力，以批判性地评估和改进 表面等离子体共振的设计和分析方法 (SPR)实验用于研究蛋白质：蛋白质相互作用。 固相共振法为测定大分子动力学提供了有力的手段。 相互作用时，实验设置通常意味着测量数据 偏离了基于基本动力学模型的预测。这些 偏差可能会导致对实验数据的错误分析。这个 偏差可能来自多种来源，如质量传输和 重新绑定效果。目前建议在以下条件下进行动力学实验 导致低信噪比的条件，这可能会导致进一步 数据分析方面的困难。它被假设应用于 在信号处理文献中已经开发的技术可以 解决一些存在的问题。本申请的目的是：1) 为了研究参考细胞减影和基线漂移对 测量的动力学常数，并开发出克服 潜在的问题；2)开发方法以改进现有的 处理质量传输、重新结合、低信噪比的技术 和快速脱落率；3)开发进一步的方法来识别 修正给定蛋白质的相互作用模型：蛋白质相互作用；以及4) 开发一个软件环境，在其中可以有效地进行这些研究 被执行。

DESCRIPTION: (Adapted from the applicant's abstract) This application represents an interdisciplinary effort to critically evaluate and improve on current methodologies for the design and analysis of surface plasmon resonance (SPR) experiments as they are used to investigate protein:protein interactions. SPR offers a powerful technique to determine the kinetics of macromolecular interactions, the experimental set-up often implies that the measured data deviates from that predicted based on the underlying kinetic model. These deviations can lead to incorrect analysis of the experimental data. The deviations can arise from a multitude of sources, such as mass transport and rebinding effects. It is currently recommended to run kinetic experiments under conditions that result in a low signal to noise ratio which can lead to further difficulties in the data analysis. It is hypothesized that the application of techniques which have been developed in the signal processing literature can resolve some of the existing problems. The aims of this application are to: 1) to investigate the effects of reference cell subtraction and baseline drift on the measured kinetic constants and to develop methods that overcome the potential problems; 2) to develop methods to improve on the currently available techniques to deal with mass transport, rebinding, low signal to noise ratios and fast off rates; 3) to develop further methods for the identification of the correction interaction model for a given protein:protein interaction; and 4) to develop a software environment in which these studies can be efficiently carried out.