Maintaining our Atmosphere - Nitrogen Cycling Enzymes of Anaerobic Ammonium Oxidation

维持我们的大气 - 厌氧氨氧化氮循环酶

• 批准号: 1654436
• 金额: --
• 依托单位: University of East Anglia
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1654436
• 关键词: Maintaining; our; Atmosphere; Nitrogen; Cycling

Anaerobic ammonium oxidation or 'anammox' is the most recent addition to processes recognised in the biogeochemical nitrogen cycle (1,2). Originally discovered in a Dutch waste-water treatment plant, anammox bacteria combine nitrite and ammonium to make N2 in the absence of O2. It has since become clear that these bacteria are metabolically dominant in O2-minimum zones across the world's oceans and may produce one out of every two N2 molecules released annually into the atmosphere. Despite the environmental and biotechnological importance of anammox there is very little knowledge of the enzymology underpinning the process. This exciting and ambitious project builds on recent success in the purification of novel anammox enzymes and has the aim of resolving the structural, catalytic and redox properties of these enzymes (1-5). The successful applicant will be a talented biochemist or chemist with an enthusiasm for metalloproteins and enzymology who will develop advanced skills in the characterisation of redox enzymes by spectroscopic (EPR and MCD), spectro-electrochemical and protein film voltammetric methods. Research will be performed within the vibrant Centre for Molecular and Spectroscopic Biochemistry under the supervision of Professor Julea Butt and Dr Myles Cheesman in the School of Chemistry, University of East Anglia and in collaboration with Dr Jan Keltjens, Dr Boran Kartal and Prof Mike Jetten, Nijmegen University, NL.

厌氧氨氧化或‘厌氧氨氧化’是在生物地球化学氮循环(1，2)中确认的最新添加过程。厌氧氨氧化细菌最初是在荷兰的一家废水处理厂发现的，它在没有氧气的情况下结合亚硝酸盐和铵生成氮气。自那以后，很明显，这些细菌在全球海洋中氧气最低的区域代谢占主导地位，每年释放到大气中的每两个氮气分子中可能就有一个产生。尽管Anammox在环境和生物技术上具有重要意义，但人们对支撑这一过程的酶学知识知之甚少。这个令人兴奋和雄心勃勃的项目建立在最近在Anammox酶的纯化方面取得的成功的基础上，目的是解决这些酶的结构、催化和氧化还原性质(1-5)。成功的申请者将是一位对金属蛋白和酶学充满热情的有才华的生物化学家或化学家，他们将发展通过光谱(EPR和MCD)、光谱电化学和蛋白质膜伏安方法表征氧化还原酶的高级技能。研究将在充满活力的分子和光谱生物化学中心内进行，由东安格利亚大学化学学院的Julea Butt教授和Myles Cheesman博士监督，并与NL奈梅亨大学的Jan Keltjens博士、Boran Kartal博士和Mike Jetten教授合作进行。