STUDY OF LDL OXIDIZABILITY IN THE NCEP PLUS DIET

这项研究提案的广泛、长期目标是检查NCEP/AHA Step One饮食目前是否因过度强调避免脂肪和胆固醇而低估了植物性食品的包含对降低冠心病(CHD)风险的潜在影响。氧化应激，根据低密度脂蛋白的氧化性，是动脉粥样硬化潜能的一个重要决定因素。该研究的具体目的是检测以植物为基础的NCEP/AHA第一步饮食中成年人的低密度脂蛋白氧化能力，以及服用商业上可获得的减脂产品的成年人的低密度脂蛋白的氧化能力。在一项采用平行设计的临床试验中，70名低密度脂蛋白-胆固醇(LDL-C130-190 mg/dL)中度升高的成年人被随机分成两种饮食之一，为期四周。提供了所有的膳食，并通过化学分析确定了两种研究饮食的组成。申请者将通过测量铜++诱导的共轭二烯形成的滞后阶段来检查两组在研究结束时获得的血清样本中低密度脂蛋白对氧化的敏感性。

The broad, long term objective of the research proposal is to examine whether the potential impact on coronary heart disease (CHD) risk reduction of an NCEP/AHA Step One Diet may be currently underestimated because of an overemphasis on the avoidance of fat and cholesterol and an underemphasis on the inclusion of plant-based foods. Oxidative stress, as estimated by LDL oxidizability, is an important determinant of atherosclerotic potential. The specific aims are to examine LDL oxidizability in adults on a plant based NCEP/AHA Step One Diet versus adults on a diet of commercially available reduced fat products. In a clinical trial using parallel design, seventy adults with moderately elevated LDL-cholesterol (LDL-C 130-190 mg/dL) were randomized to one of the two diets for four weeks. All meals were provided, and the composition of the two study diets were determined by chemical analysis. The applicant will examine the susceptibility of LDL to oxidation in serum samples obtained at the end of the study period in both groups by measuring the lag phase of conjugated diene formation induced by Copper++.