SHARED ELECTRON MICROSCOPE WITH CRYO-CAPACITY

具有低温功能的共享电子显微镜

• 批准号: 6051614
• 负责人: MARK j YOUNG
• 金额: $ 32.16万
• 依托单位: MONTANA STATE UNIVERSITY - BOZEMAN
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2002-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6051614
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; cryoelectron microscopy

Funds are requested to acquire a state-of-the-art transmission electron microscope (TEM) with cryo capacity. This instrument package will be used by a diverse group of highly productive scientists form the departments of Chemistry and Biochemistry, Microbiology, Biology, Veterinary and Molecular Biology, and Plant Sciences. During the past 10 years there has been a five-fold increase in the research activity at the Montana State University-Bozeman campus. Concatenate with this increase has been an increase in demand for TEM time and capacity. During the past 6 years there has been a three-fold increase in demand for biological TEM services. Unfortunately we are currently not able to meet the demand for TEM services. Moreover, the existing TEM capacity is nearly 20 years old and lacks cryo ability which cannot be upgraded. The two significant advantages of a new cryo TEM facility to the MSU research community are 1) to provide a much needed expanded capacity to perform cryo TEM for numerous biological applications 2) to provide a robust and reliable TEM capacity for a rapidly expanding MSU research community. The specific advantages of the proposed cryo TEM package include: Cryo-fixation of the specimens allows its structure and chemical composition to be maintained close to its natural state. Expanded imaging capacity to include CCD-based digital imaging and direct imaging through the projection chamber's fluorescent screen and platefilm. The ability to further expand the TEM capacity to include spectrum profiling and spectrum imaging (STEM, EDS, and EELS detectors and energy filters). In summary, the acquisition of a state-of-the-art TEM with cryo capacity will directly impact the research of the investigators included in this proposal, as well as a large group of secondary investigators from departments across the MSU campus. This will enable these outstanding researchers to keep pace with the expanding use of cryo-TEM in biological research.

需要资金来购买具有冷冻能力的最先进的透射电子显微镜（TEM）。该仪器包将由化学和生物化学、微生物学、生物学、兽医和分子生物学以及植物科学系的众多高产科学家使用。过去 10 年里，蒙大拿州立大学博兹曼校区的研究活动增加了五倍。与这种增长相伴随的是对 TEM 时间和容量的需求的增加。在过去 6 年里，对生物 TEM 服务的需求增长了三倍。不幸的是，我们目前无法满足 TEM 服务的需求。而且，现有的TEM能力已有近20年历史，缺乏冷冻能力，无法升级。对于密歇根州立大学研究界来说，新的冷冻 TEM 设施有两个显着优势：1) 为众多生物应用提供急需的冷冻 TEM 扩展能力，2) 为快速扩张的密歇根州立大学研究界提供强大而可靠的 TEM 能力。所提出的冷冻 TEM 套件的具体优点包括： 样本的冷冻固定使其结构和化学成分保持接近其自然状态。成像能力得到扩展，包括基于 CCD 的数字成像和通过投影室的荧光屏和底片的直接成像。能够进一步扩展 TEM 能力，包括光谱分析和光谱成像（STEM、EDS 和 EELS 探测器和能量过滤器）。总之，购买具有低温能力的最先进的 TEM 将直接影响本提案中的研究人员以及来自密歇根州立大学校园各部门的一大批二级研究人员的研究。这将使这些杰出的研究人员能够跟上冷冻 TEM 在生物研究中的广泛应用。