SHARED ELECTRON MICROSCOPE WITH CRYO-CAPACITY

需要资金购买具有低温能力的最先进的透射式电子显微镜。该仪器包将由来自化学和生物化学、微生物学、生物学、兽医和分子生物学以及植物科学系的一群高生产率的科学家使用。在过去的10年里，蒙大拿州立大学博兹曼分校的研究活动增加了五倍。随之而来的是对瞬变电磁时间和容量的需求增加。在过去的6年里，对生物瞬变电磁服务的需求增加了三倍。遗憾的是，我们目前无法满足对瞬变电磁服务的需求。此外，现有的瞬变电磁能力已有近20年的历史，缺乏低温能力，无法升级。对于密歇根州立大学的研究界来说，新的低温瞬变电磁设施的两个显著优势是：1)为许多生物应用提供急需的扩展能力，2)为迅速扩张的密歇根州立大学研究界提供强大而可靠的瞬变电磁能力。建议的冷冻电子显微镜组件的具体优点包括：冷冻固定使样品的结构和化学成分保持接近其自然状态。扩展成像能力，包括基于电荷耦合器件的数字成像和通过投影室的荧光屏和平板胶片直接成像。能够进一步扩展瞬变电磁法的容量，以包括光谱剖析和光谱成像(STEM、EDS和EELS探测器和能量过滤器)。总而言之，收购具有低温能力的最先进的瞬变电磁将直接影响本提案中包括的调查人员的研究，以及密歇根州立大学校园各部门的一大批二级调查人员的研究。这将使这些杰出的研究人员跟上低温电子显微镜在生物研究中不断扩大的使用步伐。

Funds are requested to acquire a state-of-the-art transmission electron microscope (TEM) with cryo capacity. This instrument package will be used by a diverse group of highly productive scientists form the departments of Chemistry and Biochemistry, Microbiology, Biology, Veterinary and Molecular Biology, and Plant Sciences. During the past 10 years there has been a five-fold increase in the research activity at the Montana State University-Bozeman campus. Concatenate with this increase has been an increase in demand for TEM time and capacity. During the past 6 years there has been a three-fold increase in demand for biological TEM services. Unfortunately we are currently not able to meet the demand for TEM services. Moreover, the existing TEM capacity is nearly 20 years old and lacks cryo ability which cannot be upgraded. The two significant advantages of a new cryo TEM facility to the MSU research community are 1) to provide a much needed expanded capacity to perform cryo TEM for numerous biological applications 2) to provide a robust and reliable TEM capacity for a rapidly expanding MSU research community. The specific advantages of the proposed cryo TEM package include: Cryo-fixation of the specimens allows its structure and chemical composition to be maintained close to its natural state. Expanded imaging capacity to include CCD-based digital imaging and direct imaging through the projection chamber's fluorescent screen and platefilm. The ability to further expand the TEM capacity to include spectrum profiling and spectrum imaging (STEM, EDS, and EELS detectors and energy filters). In summary, the acquisition of a state-of-the-art TEM with cryo capacity will directly impact the research of the investigators included in this proposal, as well as a large group of secondary investigators from departments across the MSU campus. This will enable these outstanding researchers to keep pace with the expanding use of cryo-TEM in biological research.