INVERTED MICROSCOPE WITH CONFOCAL ATTACHMENT

带共焦附件的倒置显微镜

• 批准号: 6051792
• 负责人: HENRY SHUMAN
• 金额: $ 18.76万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6051792
• 关键词: biomedical equipment purchase; confocal scanning microscopy; lung disorder; oxidative stress

This proposal is for the purchase of a Bio-Rad MicroRadiance confocal laser scanning microscope system including a Nikon Eclipse TE300 inverted microscope as a multi-user shared instrument. The confocal microscope will be located in and maintained by the Institute for Environmental Medicine under the supervision of the PI. Operational and maintenance costs will be recovered from an annual contribution from the users, and technical support will be provided from the Institute's operational budget. The common scientific thread in the research measures, and treatment of acute lung diseases in the newborn and the adult. The projects involve study of generation of tissue- damaging oxidants and their regulation, cell adhesion molecule function, genetic and pathophysiologic regulation of surfactant proteins and lipids, signal transduction and cell-cell communication. The projects will use isolated rat and mouse lungs, cultured type II and smooth muscle cells from rat lungs and other available lung cell lines, human fetal lung explants, leukocytes and rat brain blood vessels. These studies, often in live cells, thick explant tissue, or isolated lung, require confocal microscopic techniques using fluorescence probes for visualization of cell biological processes. The investigators participating in this proposal carry out research requiring cytological localization and quantification of molecules pertinent to their fields of study, namely: surfactant phospholipid trafficking (Shuman), assessment of endothelial dysfunction in lung ischemic injury (Fisher), evaluation of cellular distribution and function of PECAM-1 (Albelda), determination of the cellular and subcellular sites of surfactant protein SP-C processing (Beers), study of distribution of ICAM and nitrotyrosine in CO-poisoning (Thom), assess distribution of C/EBP and proSP-C (Feinstein), study of trafficking of SP-B intermediates and GPF (Guttentag), evaluation of cytoskeletal and signal transduction proteins in tracheal smooth muscle cells (Coburn), study of cellular distribution of PECAM-1, GOX and catalase (Muzykantov), and determination of colocalization and trafficking of gap junction proteins connexins 43 and 26 (Koval). These projects which are currently supported by 3,172,240 dollars per annum in total costs from NIH will be greatly facilitated by the greater availability of a confocal microscope.

该提案旨在购买 Bio-Rad MicroRadiance 共焦激光扫描显微镜系统，包括 Nikon Eclipse TE300 倒置显微镜作为多用户共享仪器。 共聚焦显微镜将位于环境医学研究所并在 PI 的监督下进行维护。 运营和维护费用将从用户的年度捐款中回收，技术支持将从研究所的运营预算中提供。 新生儿和成人急性肺部疾病的研究措施和治疗的共同科学主线。 这些项目涉及组织损伤性氧化剂的产生及其调节、细胞粘附分子功能、表面活性蛋白和脂质的遗传和病理生理调节、信号转导和细胞间通讯的研究。 这些项目将使用离体的大鼠和小鼠肺、培养的大鼠肺 II 型细胞和平滑肌细胞以及其他可用的肺细胞系、人胎儿肺外植体、白细胞和大鼠脑血管。 这些研究通常在活细胞、厚外植体组织或离体肺中进行，需要使用荧光探针的共焦显微技术来可视化细胞生物过程。 参与该提案的研究人员开展了需要对与其研究领域相关的分子进行细胞学定位和定量的研究，即：表面活性剂磷脂运输（S human）、肺缺血性损伤中内皮功能障碍的评估（Fisher）、PECAM-1 的细胞分布和功能的评估（Albelda）、确定 表面活性蛋白 SP-C 加工 (Beers)、一氧化碳中毒中 ICAM 和硝基酪氨酸分布的研究 (Thom)、评估 C/EBP 和 proSP-C 的分布 (Feinstein)、SP-B 中间体和 GPF 运输的研究 (Guttentag)、气管平滑肌细胞中细胞骨架和信号转导蛋白的评估 (Coburn)、细胞分布研究 PECAM-1、GOX 和过氧化氢酶 (Muzykantov) 的分析，以及间隙连接蛋白连接蛋白 43 和 26 (Koval) 的共定位和运输的测定。这些项目目前由 NIH 每年提供 3,172,240 美元的总成本支持，共聚焦显微镜的可用性将大大促进这些项目的发展。