MOLECULAR IMAGING UNIT

分子成像装置

• 批准号: 6053819
• 负责人: Thomas J. Murphy
• 金额: $ 9万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6053819
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; image processing; microarray technology

As a group, the twelve investigators supervise vigorous research programs, share complementary research interests, and are amply supported by MH mechanisms. The design of this Molecular Imaging Unit is composed of three synergistic modules of equipment. The combination of these modules fully exploits the dramatic convergence of imaging technologies with the analytical methods used to study the regulation of cellular function and gene expression patterns. As the various world-wide Genome Initiatives are nearing fruition, we recognize unique opportunities to examine the significance of individual research problems on sub-genomic, and eventually genomic, scales. The Molecular Imaging Unit will both enhance our current research activities and facilitate our entry into this new and exciting direction of biomedical research. The first module is a Molecular Dynamics Storm 860 Phosphorimager/Fluorimager system, which will enhance data acquisition and quantitative analysis of experiments based upon protein and nucleic acid blots and gels. This module both fills a glaring gap in our present analytical capacity and will expand the utility of the additional modules in the Unit. The second module is a General Scanning Inc. ScanArray-5000 microarray slide reader, which will allow for comprehensive and quantitative analysis of gene expression patterns using gene microarray technology. The Storm 860 system will prove to be an, indispensable tool for full exploitation of the vast amount of information that will be collected by our investigators using microarray analysis. The third module is an Arcturus Laser Capture Microdissection PixCell II system. This device is used to extract individual cells or clusters of cells from complex tissues. Protein and nucleic acid content within these cells can then be subjected to further molecular analysis at either a sub- genome wide level, using microarray analysis, or to examine the modulation of specific molecules in the cells using the Storm 860 system.

作为一个小组，12名研究人员监督积极的研究项目，分享互补的研究兴趣，并得到MH机制的充分支持。该分子成像单元的设计由三个协同模块的设备组成。这些模块的结合充分利用了成像技术与用于研究细胞功能和基因表达模式调节的分析方法的戏剧性融合。随着各种世界范围的基因组计划即将取得成果，我们认识到有独特的机会在亚基因组，并最终在基因组范围内研究个体研究问题的重要性。分子成像组将加强我们目前的研究活动，并促进我们进入这一令人兴奋的生物医学研究新方向。第一个模块是分子动力学风暴860磷光成像仪/荧光成像仪系统，它将加强基于蛋白质和核酸斑点和凝胶的实验的数据采集和定量分析。这一模块既填补了我们目前分析能力中的一个明显空白，又将扩大该股其他模块的效用。第二个模块是General Scanning Inc.ScanArray-5000微阵列幻灯片阅读器，它将允许使用基因微阵列技术对基因表达模式进行全面和定量的分析。Storm 860系统将被证明是充分利用我们的研究人员将使用微阵列分析收集的海量信息的不可或缺的工具。第三个模块是Arcturus激光捕获显微切割PixCell II系统。这种设备用于从复杂的组织中提取单个细胞或成群的细胞。然后，可以使用微阵列分析在亚基因组水平上对这些细胞中的蛋白质和核酸含量进行进一步的分子分析，或者使用Storm 860系统来检查细胞中特定分子的调节。