ABI PRISM 377 DNA SEQUENCER

ABI PRISM 377 DNA 测序仪

• 批准号: 6054058
• 负责人: DIANNA M MILEWICZ
• 金额: $ 16.63万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6054058
• 关键词: biomedical equipment purchase; computer assisted sequence analysis; nucleic acid sequence

The aim of our proposal is to establish a Genomics Core Facility for high throughput genotyping and DNA genomics-based clinical research in the Department of Internal Medicine and foster collaborations to study the genetic basis of complex diseases by other investigators in the Department. In addition, the core facility will be used for pilot projects proposed by junior clinical investigators through the University Clinical Research Center. We are applying for funds to purchase an ABI Prism 377 DNA Sequencer for this Genomic Core Facility. Currently the Department of Internal Medicine has one ABI Prism 310 DNA Genetic Analyzer, which is being used around the clock by researchers within the Department. The users of this machine are primarily the P.I. and collaborating investigators, and they average 1400 runs per month, primarily for genotype analysis. The average waiting period to analyze samples is 4 weeks because of the intensive use of the machine. The ABI 310 is the only sequencer available for genotype analysis within The University of Texas-Houston Medical School. Increased usage is projected based on the proposed aims of currently funded projects of the collaborating investigators. Ten percent of the time the ABI Prism 377 Sequencer will be reserved for junior investigators initiating pilot studies involving genomics-based clinical research through the University Clinical Research Center. Therefore, addition of the ABI Prism 377 DNA Sequencer would significantly enhance ongoing and future genomics- based clinical research within the University of Texas-Houston Medical School.

我们提案的目的是在内科建立一个基因组学核心设施，用于高通量基因分型和基于 DNA 基因组学的临床研究，并促进该科其他研究人员合作研究复杂疾病的遗传基础。此外，核心设施将用于初级临床研究人员通过大学临床研究中心提出的试点项目。我们正在申请资金为该基因组核心设施购买 ABI Prism 377 DNA 测序仪。目前内科拥有一台ABI Prism 310 DNA基因分析仪，供内科研究人员全天候使用。 这台机器的用户主要是 P.I.和合作研究人员，他们平均每月运行 1400 次，主要用于基因型分析。由于机器的频繁使用，分析样品的平均等待时间为 4 周。 ABI 310 是德克萨斯大学休斯顿分校医学院唯一可用于基因型分析的测序仪。根据合作研究人员目前资助的项目的拟议目标，预计使用量会增加。百分之十的情况下，ABI Prism 377 测序仪将保留给初级研究人员，通过大学临床研究中心启动涉及基于基因组学的临床研究的试点研究。因此，添加 ABI Prism 377 DNA 测序仪将显着增强德克萨斯大学休斯顿分校医学院正在进行和未来基于基因组学的临床研究。