ANALYTICAL ULTRACENTRIFUGE EQUIPMENT PURCHASE

本申请的目标是获得新型贝克曼分析超速离心机和附件，用于建立分析大分子的设施，大分子组合之间和大分子组合内部的相互作用通常是理解许多具有医学重要性的复杂细胞过程的关键。由 8 名研究人员、25 名研究生和 10 名博士后研究员组成的小组将组成最初的用户组，研究多亚基蛋白质组装、配体依赖性自缔合过程、其他蛋白质-蛋白质相互作用、蛋白质-核酸相互作用和胶束-胶束相互作用。该设施将成为该大学细胞和分子生物学研究所已建立的蛋白质微量分析核心设施的一部分。超速离心机将成为生物物理化学研究生和博士后研究和培训的重要工具。

The goal of this application is to acquire a new Beckman analytical ultracentrifuge and accessories to be used to establish a facility for the analysis of macromolecules and their interactions between and within macromolecular assemblages are frequently the key to understanding many complex cellular processes of medical importance. A group of 8 investigators with 25 graduate students and 10 post-doctoral fellows will form the initial user group in studies of multi-subunit protein assembly, ligand-dependent self-association processes, other protein-protein interactions, protein-nucleic acid interactions, and micelle-micelle interactions. The facility will become part of an established Protein Microanalysis Core Facility of the Institute for Cellular and Molecular Biology of the University. The ultracentrifuge will be an important tool for research and training of graduate students and postdoctoral fellows in biophysical chemistry.