ANALYTICAL ULTRACENTRIFUGE EQUIPMENT PURCHASE

本申请的目的是获得一种新的贝克曼分析超离心机和附件，用于建立大分子分析设施，大分子组合之间和内部的相互作用通常是理解许多具有医学重要性的复杂细胞过程的关键。一个由8名研究人员组成的小组，包括25名研究生和10名博士后，将在多亚基蛋白质组装、配体依赖的自结合过程、其他蛋白质-蛋白质相互作用、蛋白质-核酸相互作用和胶束-胶束相互作用的研究中形成初始用户组。该设施将成为该大学细胞和分子生物学研究所已建立的蛋白质微分析核心设施的一部分。超离心机将成为生物物理化学研究和培养研究生和博士后的重要工具。

The goal of this application is to acquire a new Beckman analytical ultracentrifuge and accessories to be used to establish a facility for the analysis of macromolecules and their interactions between and within macromolecular assemblages are frequently the key to understanding many complex cellular processes of medical importance. A group of 8 investigators with 25 graduate students and 10 post-doctoral fellows will form the initial user group in studies of multi-subunit protein assembly, ligand-dependent self-association processes, other protein-protein interactions, protein-nucleic acid interactions, and micelle-micelle interactions. The facility will become part of an established Protein Microanalysis Core Facility of the Institute for Cellular and Molecular Biology of the University. The ultracentrifuge will be an important tool for research and training of graduate students and postdoctoral fellows in biophysical chemistry.