ACQUISITION OF AN ABI 377 AUTOMATIC DNA SEQUENCE

ABI 377 自动 DNA 序列的获取

• 批准号: 6053818
• 负责人: DAVID MCKINNON
• 金额: $ 11.07万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-15 至 2001-11-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6053818
• 关键词: biomedical equipment purchase; computer assisted sequence analysis; nucleic acid sequence

This proposal requests funds for the purchase of a Perkin Elmer ABI DNA Sequencer 377 with upgrade to 96 lanes. The requested instrument package includes the automatic sequencing apparatus including gel electrophoresis unit, laser and fluorescence detection system as well as a computer (Macintosh) and sequence detection and analysis software. This system provides all the functionality required for the analysis of fluorescently labeled DNA sequences prepared by polymerase chain reaction (PCR). The instrument will be located in the State University of New York at Stony Brook (SUNY SB) DNA Sequencing Facility which is housed in the Department of Physiology and Biophysics. The sequencer will significantly promote the research objectives and laboratory productivity of fourteen distinguished scientists at SUNY SB who comprise the major users group. The sequencer will make available to these investigators the technology required for the rapid analysis of DNA sequences which is presently inadequately supported at SUNY SB and difficult to obtain from other organizations in the local area. Automatic DNA sequencing technology is far superior to manual sequencing, which most investigators have to rely upon in part or full, due to greatly increased throughput, read-length, accuracy, ease of use and the elimination of the requirement for radionucleotides. The automatic sequencer will not only facilitate currently active projects but will also open up new possibilities for investigators allowing them to tackle much larger sequencing projects than is currently feasible. The instrument will be operated and maintained by trained personnel at the SUNY SB DNA Sequencing Facility. The director of the facility will schedule daily operation and provide technical expertise for the users. An advisory committee will ensure access to the major users and other PHS- supported investigators and will be responsible for the long term operation of the instrument and the facility.

该提案要求提供资金，用于购买Perkin Elmer ABI DNA测序仪377，升级为96道。 要求的仪器包包括自动测序装置，包括凝胶电泳单元、激光和荧光检测系统以及计算机（Macintosh）和序列检测和分析软件。该系统提供了通过聚合酶链式反应（PCR）制备的荧光标记DNA序列分析所需的所有功能。该仪器将位于斯托尼布鲁克的纽约州立大学（SUNY SB）DNA测序设施，该设施位于生理学和生物物理学系。测序仪将显着促进研究目标和实验室生产力的14名杰出的科学家在纽约州立大学SB谁构成的主要用户组。测序仪将为这些研究人员提供快速分析DNA序列所需的技术，该技术目前在SUNY SB没有得到充分的支持，也很难从当地的其他组织获得。自动DNA测序技术远远上级大多数研究人员必须部分或全部依赖的手动测序，这是由于大大增加了通量、读取长度、准确性、易用性和消除了对放射性同位素的需求。自动测序仪不仅将促进目前正在进行的项目，而且还将为研究人员开辟新的可能性，使他们能够处理比目前可行的更大的测序项目。该仪器将由SUNY SB DNA测序设施的受过培训的人员操作和维护。设施主任将安排日常运作，并为用户提供技术专门知识。一个咨询委员会将确保接触主要用户和其他PHS支持的调查人员，并将负责仪器和设施的长期运作。