ACQUISITION OF AN ABI 377 AUTOMATIC DNA SEQUENCE

该提案要求提供资金，用于购买Perkin Elmer ABI DNA Sequencer 377，升级到96个通道。所要求的成套仪器包括自动测序仪器，包括凝胶电泳仪、激光和荧光检测系统以及一台计算机(Macintosh)和序列检测和分析软件。该系统提供了分析聚合酶链式反应(PCR)制备的荧光标记DNA序列所需的所有功能。该仪器将设在纽约州立大学石溪分校(SUNY SB)DNA测序设施，该设施设在生理和生物物理系。该测序仪将显著提高纽约州立大学SB分校14名杰出科学家的研究目标和实验室生产率，他们是主要用户群体。该测序仪将向这些调查人员提供快速分析DNA序列所需的技术，目前在SUNY SB没有得到充分的支持，而且很难从当地的其他组织获得。自动DNA测序技术远远优于大多数研究人员不得不部分或全部依赖的人工测序，因为大大提高了吞吐量、读数长度、准确性、易用性，并消除了对放射性核苷酸的要求。自动测序仪不仅将促进目前正在进行的项目，还将为调查人员开辟新的可能性，使他们能够处理比目前可行的更大的测序项目。该仪器将由纽约州立大学SB DNA测序设施训练有素的人员操作和维护。该设施的负责人将安排日常运营并为用户提供技术专业知识。一个咨询委员会将确保接触主要用户和其他由小灵通支助的调查人员，并负责仪器和设施的长期运作。

This proposal requests funds for the purchase of a Perkin Elmer ABI DNA Sequencer 377 with upgrade to 96 lanes. The requested instrument package includes the automatic sequencing apparatus including gel electrophoresis unit, laser and fluorescence detection system as well as a computer (Macintosh) and sequence detection and analysis software. This system provides all the functionality required for the analysis of fluorescently labeled DNA sequences prepared by polymerase chain reaction (PCR). The instrument will be located in the State University of New York at Stony Brook (SUNY SB) DNA Sequencing Facility which is housed in the Department of Physiology and Biophysics. The sequencer will significantly promote the research objectives and laboratory productivity of fourteen distinguished scientists at SUNY SB who comprise the major users group. The sequencer will make available to these investigators the technology required for the rapid analysis of DNA sequences which is presently inadequately supported at SUNY SB and difficult to obtain from other organizations in the local area. Automatic DNA sequencing technology is far superior to manual sequencing, which most investigators have to rely upon in part or full, due to greatly increased throughput, read-length, accuracy, ease of use and the elimination of the requirement for radionucleotides. The automatic sequencer will not only facilitate currently active projects but will also open up new possibilities for investigators allowing them to tackle much larger sequencing projects than is currently feasible. The instrument will be operated and maintained by trained personnel at the SUNY SB DNA Sequencing Facility. The director of the facility will schedule daily operation and provide technical expertise for the users. An advisory committee will ensure access to the major users and other PHS- supported investigators and will be responsible for the long term operation of the instrument and the facility.