ACQUISITION OF AN ABI 377 AUTOMATIC DNA SEQUENCE

本提案请求资金用于购买珀金埃尔默ABI DNA测序仪377并升级到96车道。所要求的仪器包包括自动测序仪，包括凝胶电泳装置、激光和荧光检测系统以及计算机（Macintosh）和序列检测和分析软件。该系统提供了所有所需的功能，用于分析荧光标记的DNA序列制备的聚合酶链反应（PCR）。该仪器将位于纽约州立大学石溪分校（SUNY SB）生理和生物物理系的DNA测序设施。该测序仪将极大地促进SUNY SB的14位杰出科学家的研究目标和实验室生产力，他们构成了主要用户群。测序仪将为这些研究人员提供快速分析DNA序列所需的技术，目前在纽约州立大学SB分校得不到足够的支持，而且很难从当地的其他组织获得。自动DNA测序技术远远优于大多数研究人员不得不部分或全部依赖的人工测序，因为它大大增加了通量，读取长度，准确性，易用性和消除了对放射性核苷酸的要求。自动测序仪不仅将促进目前正在进行的项目，而且还将为研究人员开辟新的可能性，使他们能够处理比目前可行的更大的测序项目。仪器将由纽约州立大学SB分校DNA测序中心训练有素的人员操作和维护。设施主任将安排日常操作并为用户提供技术专业知识。一个咨询委员会将确保能够接触到主要用户和其他小灵通支助的调查人员，并将负责仪器和设施的长期运行。

This proposal requests funds for the purchase of a Perkin Elmer ABI DNA Sequencer 377 with upgrade to 96 lanes. The requested instrument package includes the automatic sequencing apparatus including gel electrophoresis unit, laser and fluorescence detection system as well as a computer (Macintosh) and sequence detection and analysis software. This system provides all the functionality required for the analysis of fluorescently labeled DNA sequences prepared by polymerase chain reaction (PCR). The instrument will be located in the State University of New York at Stony Brook (SUNY SB) DNA Sequencing Facility which is housed in the Department of Physiology and Biophysics. The sequencer will significantly promote the research objectives and laboratory productivity of fourteen distinguished scientists at SUNY SB who comprise the major users group. The sequencer will make available to these investigators the technology required for the rapid analysis of DNA sequences which is presently inadequately supported at SUNY SB and difficult to obtain from other organizations in the local area. Automatic DNA sequencing technology is far superior to manual sequencing, which most investigators have to rely upon in part or full, due to greatly increased throughput, read-length, accuracy, ease of use and the elimination of the requirement for radionucleotides. The automatic sequencer will not only facilitate currently active projects but will also open up new possibilities for investigators allowing them to tackle much larger sequencing projects than is currently feasible. The instrument will be operated and maintained by trained personnel at the SUNY SB DNA Sequencing Facility. The director of the facility will schedule daily operation and provide technical expertise for the users. An advisory committee will ensure access to the major users and other PHS- supported investigators and will be responsible for the long term operation of the instrument and the facility.