HIGH RESOLUTION CONFOCAL MICROSCOPY IN LIVING CELLS

该项目的目标是在西北医学院建立一个多用户设施，该设施将允许在活细胞中进行高速共聚焦显微镜检查，在活细胞中可以保持生理条件并进行细胞电生理测量。该设施旨在满足多名研究人员的需求，这些研究人员由NIH资助赠款并且需要所需仪器的能力，以便进一步实现其正在进行的研究的特定目标。目前，每个实验室分别提供了这种技术中可能采用的方法。医学院正在提供广泛的财政支助，费用分摊安排表明了这一点，其中包括共计210 250美元，用于翻修专用场地和支付5年的部分业务费用。该设施最初将支持七个独立的NIH资助的研究项目的具体目标。这些项目有一个共同的目标，即研究细胞信号传导的调节，包括在完整细胞中测量细胞内Ca、特定蛋白质的靶向和定位、蛋白质-蛋白质相互作用和分泌调节。大多数实验将在尽可能保持正常生理状态的条件下进行。迄今为止，用户组的个别成员的进展已被限制的无法测量亚细胞事件的基础细胞将成为可能，现在将允许先进的共聚焦成像技术的应用，直接研究的电生理特性和跨膜信号转导事件的细胞内调节细胞功能的关系。此外，该设施将大大加强对学生和博士后研究员的研究培训，也将对临床科学家的研究培训产生重大影响。因此，该设施很可能为医学院的研究和教学任务做出重大贡献。

The goal of this project is to establish a multi-user facility at Northwestern Medical School that will allow high speed confocal microscopy in living cells where physiological conditions can be maintained and cellular electrophysiological measurements can be made. This facility is designed to meet the needs of multiple investigators who are supported by funded NIH grants and who need the capabilities of the requested instrument in order to further the specific aims of their ongoing research. The approaches made possible in this techniques currently available in each lab separately. Extensive financial support is being provided by the Medical School as demonstrated by a cost-sharing arrangement which includes a total of $210,250 both for renovations of dedicated space and for partial operating costs for a period of 5 years. This facility initially will support the specific aims of seven separate NIH-funded research projects. These projects share a common goal of investigating regulation of cell signaling, including measurements in intact cells of intracellular Ca, targeting and localization of specific proteins, protein-protein interactions and regulation of secretion. Most experiments will be performed under conditions intended to maintain as normal a physiological state as possible. To date, progress by individual members of the user group has been limited by the inability to measure subcellular events underlying cell will be made possible will now allow the application of advanced confocal imaging technology to the direct study of the relationships of electrophysiological properties and transmembrane signaling events to the intracellular regulation of cell function. In addition, this facility will greatly enhance the research training on students and post-doctoral fellows and will also have a significant impact on the research training of clinician scientists. Consequently, this facility is likely to make a major contribution to both the research and teaching missions of the Medical School.