ABI PRISM 7700 SEQUENCE DETECTOR

本申请的目的是为圣刘易斯大学健康科学中心的多名研究者共享ABI PRISM 7700序列检测器获得资金。该仪器将使这些研究人员能够将最近开发的“真实的时间PCR”技术应用于他们目前资助的NIH项目。 该设备将PCR和荧光监测的灵敏度与核酸靶扩增的动态测量相结合。 通过复杂的计算机软件与热循环仪和荧光计集成到这台机器中，生物医学研究的新方法成为可能。这种新技术的主要优点是：1）能够进行标准PCR机器不可能进行的高度定量PCR测定，2）高通量，允许快速筛选数百至数千个样品，以及3）同时测量多达6种不同分子靶标的表达。在圣刘易斯大学或其附属机构，目前没有任何设备可以为生物医学研究提供这些革命性的新能力。该设备将由目前资助的NIH赠款的至少21名不同的主要研究者使用，主要任命在圣刘易斯大学健康科学中心的4个不同部门。

The purpose of this application is to obtain funding for an ABI PRISM 7700 Sequence Detector to be shared by multiple investigators at Saint Louis University Health Sciences Center. This instrument will make it possible for these investigators to apply the recently developed technology of "Real Time PCR" to their currently funded NIH projects. This equipment combines the sensitivities of PCR and fluorescence monitoring with dynamic measurements of nucleic acid target amplification. Through sophisticated computer software integrated with the thermocycler and fluorometer built into this machine, new approaches to biomedical research become available. The major advantages of this new technology are I) the ability to perform highly quantitative PCR assays not possible with standard PCR machines, 2) high though put allowing rapid screening of hundreds to thousands of samples, and 3) simultaneous measurements of the expression of up to 6 different molecular targets. There is no existing equipment at Saint Louis University or its affiliated institutions that presently provide these revolutionary new capacities for biomedical research. This equipment will be used by 'at least 21 different principal investigators of currently funded NIH grants with primary appointments in 4 different Departments at Saint Louis University Health Sciences Center.