A MULTI-FREQUENCY EPR SPECTROMETER

电子顺磁共振(EPR)谱是表征生物样品的一种基本技术，它提供了形成许多酶活性中心的顺磁中心(金属离子和自由基)的身份、氧化态、自旋状态和电子相互作用的基本信息。俄勒冈州研究生院(OGI)正在进行的研究需要最先进的EPR仪器，其生物生化小组在生物无机光谱学方面的成就在国际上是公认的。OGI现有的多用户EPR仪器不再适用，迫切需要新的仪器来更换老化的设备，并扩展现有的能力，以满足不断扩大的研究需求。这里要求的多频率电子顺磁共振仪器是表征金属酶和生物自由基所必需的，这是主要用户群体实验室研究的重点，是对其他光谱方法的补充。总部设在该设施的EPR协作室将与一个国际协作者小组建立联系，加强交流并促进科学互动。主要用户群体中的光谱专业知识也将提供给其他用户，使EPR设施成为一个关键资源，将延续OGI共享仪器的长期传统，支持和促进广泛的区域、国家和国际生物医学研究。

Electron paramagnetic resonance (EPR) spectroscopy is a fundamental technique for characterization of biological samples, providing essential information on the identity, oxidation state, spin state and electronic interactions of paramagnetic sites (metal ions and free radicals) that form the active sites of many enzymes. State-of-the-art EPR instrumentation is needed for ongoing research at the Oregon Graduate institute (OGI), whose Metallobiochemistry group is internationally recognized for accomplishments in bioinorganic spectroscopy. The existing multi-user EPR instrumentation at OGI is no longer serviceable, raising a critical need for new instrumentation to replace aging equipment and extend the available capabilities to meet the demands of expanding research. The multi-frequency EPR instrumentation requested here is required for characterization of metalloenzymes and biological free radicals that are the focus of research in the labs of the major user groups, complementing other spectroscopic approaches. An EPR collaboratory based in this facility will link with an international group of collaborators, enhancing communication and stimulating scientific interactions. The spectroscopic expertise within the major user groups will also be available to other users, making this EPR facility a key resource that will continue a long tradition of shared instrumentation at OGI, supporting and promoting a broad spectrum of regional, national, and international biomedical research.