A MULTI-FREQUENCY EPR SPECTROMETER

多频 EPR 光谱仪

• 批准号: 6053076
• 负责人: JAMES W WHITTAKER
• 金额: $ 19.99万
• 依托单位: OREGON GRADUATE INSTITUTE SCIENCE & TECH
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6053076
• 关键词: biomedical equipment purchase; cryoscience; electron spin resonance spectroscopy

Electron paramagnetic resonance (EPR) spectroscopy is a fundamental technique for characterization of biological samples, providing essential information on the identity, oxidation state, spin state and electronic interactions of paramagnetic sites (metal ions and free radicals) that form the active sites of many enzymes. State-of-the-art EPR instrumentation is needed for ongoing research at the Oregon Graduate institute (OGI), whose Metallobiochemistry group is internationally recognized for accomplishments in bioinorganic spectroscopy. The existing multi-user EPR instrumentation at OGI is no longer serviceable, raising a critical need for new instrumentation to replace aging equipment and extend the available capabilities to meet the demands of expanding research. The multi-frequency EPR instrumentation requested here is required for characterization of metalloenzymes and biological free radicals that are the focus of research in the labs of the major user groups, complementing other spectroscopic approaches. An EPR collaboratory based in this facility will link with an international group of collaborators, enhancing communication and stimulating scientific interactions. The spectroscopic expertise within the major user groups will also be available to other users, making this EPR facility a key resource that will continue a long tradition of shared instrumentation at OGI, supporting and promoting a broad spectrum of regional, national, and international biomedical research.

电子顺磁共振（EPR）光谱是表征生物样品的一项基本技术，提供了关于形成许多酶活性位点的顺磁位点（金属离子和自由基）的身份、氧化态、自旋态和电子相互作用的基本信息。俄勒冈研究生院（OGI）正在进行的研究需要最先进的EPR仪器，该研究所的金属生物化学小组在生物无机光谱学方面取得了国际公认的成就。OGI现有的多用户EPR仪器不再可用，这就迫切需要新的仪器来取代老化的设备，并扩展现有的能力，以满足不断扩大的研究需求。本文要求的多频EPR仪器用于金属酶和生物自由基的表征，这是主要用户群体实验室研究的重点，补充了其他光谱方法。设在该设施的EPR合作实验室将与一个国际合作者小组联系起来，加强交流并促进科学互动。主要用户群体中的光谱专业知识也将提供给其他用户，使该EPR设施成为一个关键资源，将继续OGI共享仪器的悠久传统，支持和促进广泛的区域、国家和国际生物医学研究。