A MULTI-FREQUENCY EPR SPECTROMETER

多频 EPR 光谱仪

• 批准号: 6053076
• 负责人: JAMES W WHITTAKER
• 金额: $ 19.99万
• 依托单位: OREGON GRADUATE INSTITUTE SCIENCE & TECH
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6053076
• 关键词: biomedical equipment purchase; cryoscience; electron spin resonance spectroscopy

Electron paramagnetic resonance (EPR) spectroscopy is a fundamental technique for characterization of biological samples, providing essential information on the identity, oxidation state, spin state and electronic interactions of paramagnetic sites (metal ions and free radicals) that form the active sites of many enzymes. State-of-the-art EPR instrumentation is needed for ongoing research at the Oregon Graduate institute (OGI), whose Metallobiochemistry group is internationally recognized for accomplishments in bioinorganic spectroscopy. The existing multi-user EPR instrumentation at OGI is no longer serviceable, raising a critical need for new instrumentation to replace aging equipment and extend the available capabilities to meet the demands of expanding research. The multi-frequency EPR instrumentation requested here is required for characterization of metalloenzymes and biological free radicals that are the focus of research in the labs of the major user groups, complementing other spectroscopic approaches. An EPR collaboratory based in this facility will link with an international group of collaborators, enhancing communication and stimulating scientific interactions. The spectroscopic expertise within the major user groups will also be available to other users, making this EPR facility a key resource that will continue a long tradition of shared instrumentation at OGI, supporting and promoting a broad spectrum of regional, national, and international biomedical research.

电子顺磁共振（EPR）光谱是一种用于表征生物样品的基本技术，提供了有关构成许多酶的活性位点的参数磁位（金属离子和自由基）的身份，氧化态，自旋状态和电子相互作用的基本信息。俄勒冈研究生研究所（OGI）的持续研究需要最先进的EPR仪器，其金属生物化学组因生物无机光谱学成就而受到国际认可。 OGI现有的多用户EPR仪器不再可用，这增加了对新仪器的迫切需求，以替换老化设备并扩展可用功能，以满足扩大研究的需求。此处要求的多种EPR仪器是对金属酶和生物自由基的表征所必需的，这些自由基是主要用户组实验室中研究的重点，并补充了其他光谱方法。基于该设施的EPR合作组织将与国际合作者组联系，从而增强沟通并刺激科学互动。主要用户组中的光谱专业知识也将提供给其他用户，这使该EPR设施成为OGI的悠久传统，它将继续在OGI中具有悠久的共享仪器传统，并支持和促进广泛的地区，国家和国际生物医学研究。