ANALYTICAL ULTRACENTRIFUGE

要求提供资金用于购买分析超级仪器，这将反过来支持以CWRU化学系为中心的生物有机和生物物理化学的新举措。该计划包括七个在生物大分子和超分子组装方面具有专业知识的主要用户。目前，在俄亥俄州没有超级分析中心。所要求的工具将明显提高主要用户的NIH支持项目的研究能力和生产力。它还将促进化学系，医学院和CWRU工程学院以及附近机构（如克利夫兰州立大学和克利夫兰诊所基金会）的教师和学生之间的互动和合作。Barkley博士（PI）在分析超离心方面经验丰富，特别是在蛋白质-蛋白质相互作用的应用方面。主要用户代表个人（Lee，Zagorski，Jamieson）和个人团体（Barkley，Surewicz，Shainoff，Qin）。李博士是蛋白水解酶方面的专家。Zagorski和Surewicz博士是蛋白质折叠和错误折叠的专家。Surewicz博士还代表医学院的六名用户。杰米森博士是粘蛋白方面的专家Shainoff博士是凝血蛋白专家，代表克利夫兰州立大学的两名用户。秦军博士是蛋白质结构测定方面的专家，代表克利夫兰临床基金会的八名用户。主要用户将带来蛋白质-蛋白质和蛋白质-核酸相互作用的基础研究，以及蛋白质与辅基和配体的相互作用，以解决广泛生物系统中的关键结构-功能问题。所要求的文书所产生的研究小组之间的协同作用将大大促进其研究项目的进展。所要求的分析超吸收谱将用于测量分子量以及大分子缔合常数和化学计量。还需要一台打印机用于硬拷贝输出和一台密度计用于确定浮力系数。这些设备将被安置在克利夫兰结构生物学中心（CCSB）新装修的实验室中，该中心是化学系的主要分析仪器设施（MAIF）。

Funds are requested for purchasing an analytical ultracentrifuge, which will in turn support a new initiative in bioorganic and biophysical chemistry centered in the Chemistry Department at CWRU. This initiative features seven major users with expertise in biological macromolecules and supramolecular assemblies. Presently, there are no analytical ultracentrifuges in the state of Ohio. The requested instrument will clearly enhance research prowess and productivity on the NIH-supported projects of the major users. It will also stimulate interactions and collaborations among faculty and students in the Chemistry Department, the Medical School, and the College of Engineering at CWRU as well as nearby institutions, such as Cleveland State University and the Cleveland Clinic Foundation. Dr. Barkley (PI) is experienced in analytical ultracentrifugation, particularly in applications to protein-protein interactions. The major users represent individuals (Lee, Zagorski, Jamieson) and groups of individuals (Barkley, Surewicz, Shainoff, Qin). Dr. Lee is expert on proteolytic enzymes. Drs. Zagorski and Surewicz are expert on protein folding and misfolding. Dr. Surewicz also represents six users from the Medical School. Dr. Jamieson is expert on mucins. Dr. Shainoff is expert on blood clotting proteins and represents two users from Cleveland State University. Dr. Jun Qin is expert on protein structure determination and represents eight users from the Cleveland Clinic Foundation. The major users will bring fundamental studies of protein-protein and protein-nucleic acid interactions as well as interactions of proteins with prosthetic groups and ligands to bear on key structure-function questions in a wide range of biological systems. The synergism among their research groups spawned by-the requested instrument will greatly facilitate progress on their research projects. The requested analytical ultracentrifuge will be used to measure molecular weights as well as macromolecular association constants and stoichiometry. Also requested is a printer for hard copy output and a density meter for buoyancy factor determination. The equipment will be housed in a newly renovated laboratory of the Cleveland Center for Structural Biology (CCSB), which is the Major Analytical Instrument Facility (MAIF) in the Chemistry Department.