REGULATION OF ALTERNATIVE SPLICING IN MUSCLE BY ETR-3

ETR-3 对肌肉选择性剪接的调节

• 批准号: 6374836
• 负责人: Andrea Nicole Ladd
• 金额: $ 4.02万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6374836
• 关键词: RNA binding protein; RNA splicing; laboratory mouse; myocardium; myogenesis; striated muscles

The goal of this project is to understand the mechanisms that regulate alternative splicing during striated muscle development. Cardiac troponin T (cTNT) undergoes developmentally regulated alternative slicing in striated muscle, and is a useful model for cell-specific splicing regulation. cTNT pre-mRNAs contain muscle-specific splicing enhancers (MSEs), intronic elements that are required for the enhanced alternative exon inclusion that is observed in embryonic striated muscle. ETR-3, a member of a conserved family of RNA-binding proteins, binds MSEs and promotes MSE-dependent exon inclusion. ETR-3 is induced during skeletal muscle differentiation and undergoes an isoform transition during heart development. Both of these changes correlate with regulated switches in cTNT splicing. These results strongly suggest that ETR-3 is a major regulator of alternative splicing in striated muscle development. To determine the role of ETR-3 in muscle-specific alternative splicing, I will: (i) Characterize ETR-3 as a positive regulator of alternative splicing during skeletal muscle differentiation, (ii) Determine the significance of the ETR-3 isoform transition during heart development, and (iii) Determine the role of ETR-3 in coordinated regulation of alternative splicing during striated muscle development. ETR-3 is 78% identical to CUG-BP, which is proposed to mediate pathogenesis of myotonic dystrophy, an autosomal dominant genetic disorder caused by an expansion of unstable CTG repeats in the 3' UTR of the DMPK gene. Expanded DMPK transcripts accumulate in the nucleus, and are thought to affect post-transcriptional processing of other genes by disrupting function of CUG-BP. Our results suggest that ETR-3 is an equally likely candidate for mediating a trans-dominant effect in DM.

这个项目的目标是了解在横纹肌发育过程中调节选择性剪接的机制。心肌肌钙蛋白T(CTnT)在横纹肌中经历发育调节的交替切片，是细胞特异性剪接调节的有用模型。CTnT前-mRNAs含有肌肉特异性剪接增强子(MSE)，这是在胚胎横纹肌中观察到的增强选择性外显子包涵体所必需的内含子元件。ETR-3是一个保守的RNA结合蛋白家族的成员，它结合MSE并促进MSE依赖的外显子包涵体。ETR-3在骨骼肌分化过程中被诱导，并在心脏发育过程中经历同型转换。这两个变化都与cTnT剪接中的调控开关有关。这些结果有力地表明，ETR-3是横纹肌发育中选择性剪接的主要调节因子。为了确定ETR-3在肌肉特异性选择性剪接中的作用，我将：(I)将ETR-3表征为骨骼肌分化过程中选择性剪接的正向调节因子；(Ii)确定ETR-3亚型转变在心脏发育过程中的意义；以及(Iii)确定ETR-3在横纹肌发育过程中选择性剪接的协调调节中的作用。ETR-3与CUG-BP有78%的同源性，CUG-BP被认为参与强直性肌营养不良的发病机制。强直性肌营养不良是一种常染色体显性遗传病，由DMPK基因3‘非编码区不稳定的CTG重复序列扩大引起。扩展的DMPK转录本在细胞核中积累，并被认为通过扰乱CUG-BP的功能来影响其他基因的转录后处理。我们的结果表明，ETR-3同样有可能在糖尿病中发挥跨显性作用。