A SEARCH FOR NOVEL STAPHYLOCOCCUS EPIDERMIDIS ADHESINS

新型葡萄球菌表皮粘附素的研究

• 批准号: 6406673
• 负责人: MARIA Gabriela BOWDEN
• 金额: $ 4.02万
• 依托单位: TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6406673
• 关键词: Staphylococcus epidermidis; adhesin; binding sites; chemical binding; collagen; fibronectins; genetic library; microorganism culture; molecular cloning; protein sequence

The ability of Staphylococcus epidermidis to elicit disease in the host is dependent on its attachment to tissue and serum-protein covered biomaterials. In prior studies it was shown that once established onto a surface, this organism can form polysaccharide-encased biofilms. However, the elements necessary for the initial attachment step are not well characterized. I postulate that cell-surface proteins are involved in the initial phase of S. epidermidis adherence to host tissue and biomaterials. Therefore, I propose to identify and characterize the proteins necessary for S. epidermidis attachment to collagen and fibronectin. To accomplish these goals, I intend to, a) identify, clone and sequence novel S. epidermidis collagen- and fibronectin-adhesin genes, b) define the ligand binding domain within the collagen-binding protein, c) determine the adhesin binding site in collagen, and c) analyze the molecular interactions between the adhesin and collagen. An understanding of the S. epidermidis adhesins structure and their molecular interactions with their ligands may lead to the development of therapeutic agents that could prevent biomaterials infections.

表皮葡萄球菌在宿主中引发疾病的能力取决于其对组织和血清蛋白覆盖的生物材料的附着。先前的研究表明，一旦建立在表面上，这种生物体就可以形成多糖包裹的生物膜。然而，初始连接步骤所需的元件尚未得到很好的表征。我假设细胞表面蛋白参与表皮葡萄球菌粘附宿主组织和生物材料的初始阶段。因此，我建议鉴定和表征表皮葡萄球菌附着胶原蛋白和纤连蛋白所需的蛋白质。为了实现这些目标，我打算，a) 鉴定、克隆和测序新型表皮葡萄球菌胶原和纤连蛋白粘附素基因，b) 定义胶原蛋白结合蛋白内的配体结合域，c) 确定胶原蛋白中的粘附素结合位点，c) 分析粘附素和胶原蛋白之间的分子相互作用。了解表皮葡萄球菌粘附素结构及其与配体的分子相互作用可能有助于开发预防生物材料感染的治疗剂。