Cryopreservation of a Living Human Skin Equivalent

活人皮肤等效物的冷冻保存

• 批准号: 6404181
• 负责人: Paul Barth Conrad
• 金额: $ 10.14万
• 依托单位: STRATATECH CORPORATION
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-21 至 2002-09-20
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6404181
• 关键词: artificial skin; bioengineering /biomedical engineering; biomaterial compatibility; biomaterial development /preparation; cell line; chemical structure function; crosslink; cryopreservation; cytoprotection; electrical measurement; freezing; glass; histology; homologous transplantation; human tissue; intermolecular interaction; keratinocyte; organ culture; osmotic pressure; phosphates; physical property; polyhydroxy compound; temperature; tissue /organ preservation; tissue engineering; trehalose

DESCRIPTION (provided by applicant): Production of a living human skin equivalent (LSE) that is economical, mechanically durable and provides effective barrier function for the treatment of epithelial trauma (e.g., burns and diabetic lesions) is a highly prized goal of current efforts in tissue engineering. Stratatech, L.L.C., is currently commercializing various products based on the proprietary human keratinocyte cell line, NIKStm, which has been shown to differentiate into a fully stratified living skin equivalent with normal expression of histological markers. The limited shelf life and high storage costs for currently available skin equivalent products limit their therapeutic potential. We hypothesize that a non-toxic cryoprotectant system based on saccharides specifically designed to form a glass on cooling (i.e., vitrify) rather than freeze will be more effective in the preservation of skin tissue architecture and viability than current methods (i.e., treatment with glycerol). Development of highly effective cryopreservation methods for the NIKStm cell line represents an opportunity to provide the medical community with a much needed supply of off-the-shelf skin graft material with long shelf life at relatively high storage temperatures. PROPOSED COMMERCIAL APPLICATION: As the field of tissue engineering advances, the need for effective, non-toxic preservation methods for future products grows. Stratatech is developing living human skin equivalent products based on our proprietary NIKS (TM) cell line. The goal of this proposal is to develop technology based on recent advances in cryopreservation coupled with methods to introduce protectants into target cells. We hypothesize that by enhancing glass formation a non-toxic, high storage temperature preservation system can be achieved for living human skin equivalents, a model system with high therapeutic value. It is our belief that this technology will be applicable to a wide range of other tissues and organs.

描述（由申请人提供）：活体人皮肤的生产 等效（LSE），经济，机械耐用，并提供 用于治疗上皮创伤的有效屏障功能（例如，烧伤 和糖尿病病变）是目前在组织中的努力的高度珍视的目标 工程. Stratatech，L.L.C.，目前正在商业化各种产品 基于专有的人类角质形成细胞系NIKStm， 显示分化成完全分层的活体皮肤， 组织学标志物表达正常。有限的保质期和高 目前可用的皮肤等效产品的存储成本限制了它们 治疗潜力我们假设一种无毒的冷冻保护剂系统 基于专门设计用于在冷却时形成玻璃的玻璃（即， 玻璃化）而不是冷冻将更有效地保存皮肤 组织结构和生存力（即，治疗 甘油）。高效冷冻保存方法的研究进展 NIKStm细胞系为医学界提供了一个 具有非常需要的现成的皮肤移植材料的供应 在相对较高的储存温度下的寿命。 拟定商业应用： 随着组织工程领域的发展，对未来产品有效、无毒的保存方法的需求不断增长。 Stratatech正在开发基于我们专有的NIKS（TM）细胞系的活体人类皮肤等效产品。 该提案的目标是基于冷冻保存的最新进展以及将保护剂引入靶细胞的方法来开发技术。 我们假设，通过增强玻璃形成，可以实现无毒的，高储存温度的保存系统，用于活体人类皮肤等同物，具有高治疗价值的模型系统。 我们相信，这项技术将适用于广泛的其他组织和器官。