NEW APPROACHES TO THE DIAGNOSIS OF AMEBIASIS

阿米巴病诊断新方法

基本信息

  • 批准号:
    6362456
  • 负责人:
  • 金额:
    $ 29.76万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2000
  • 资助国家:
    美国
  • 起止时间:
    2000-09-15 至 2002-08-31
  • 项目状态:
    已结题

项目摘要

Amebiasis, caused by Entamoeba histolytica, is a major problem in developing countries. In developed countries, it is most common in immigrants and travelers returning from the third world, and less common but present in men who have sex with men and immunosuppressed individuals. In previously sponsored SBIR research, we developed a stool antigen test for the detection of the E. histolytica adhesin, a protein that is an effective in vitro diagnostic marker. Our test, a microwell ELISA, is more accurate than microscopy and other commercial ELISAs, and more rapid than the "gold standard" culture/zymodeme, which takes 2 weeks to complete. We now have developed antigen affinity-purified antibody that has enabled us to increase the sensitivity' of the test ten-fold. We propose to use the improved antibody in dipsticks that can be used in the field as a rapid stool antigen test. In an effort to make immunoassays available to laboratories that test only fixed specimens (where most misdiagnosis of amebiasis occurs in the U.S.), we will develop immunoassays that detect formalin-stable Entamoeba antigens. We also propose to develop an antibody-based test for Entamoeba cysts. Polyclonal and monoclonal antibodies against cysts will be developed and used in screening immunoassays for contaminated water and food (recent food and water- borne outbreaks in Reston Virginia and Tblissi, Republic of Georgia respectively, make this a topical and immediate need). The development of cyst-specific tests will be patterned after those for the cyst- and oocyst- specific antigens of Giardia and Cryptosporidium. The data collected will be used to develop 510(k) documents for submission to and review by the FDA. This project will lead to a battery of tests that have broad application in the diagnosis of amebiasis. The tests will be crucial tools for evaluation of amebiasis vaccine candidates developed under our separate Tropical Disease Research Unit. PROPOSED COMMERCIAL APPLICATIONS: New diagnostic tests will be developed for amebiasis to solve the problems of misdiagnosis at community and reference labs in the U.S., enable screening of water supplies for the parasite, and to permit field evaluation of vaccine trials. Our results should lead to commercial tests that will be of significant value to the health industry around the world.
由溶组织内阿米巴引起的阿米巴病是发展中国家的一个主要问题。在发达国家,这在从第三世界返回的移民和旅行者中最常见,较少见,但在与男性发生性关系的男性和免疫功能低下的个人中存在。在之前赞助的SBIR研究中,我们开发了一种粪便抗原检测方法,用于检测溶组织埃希菌粘附素,这是一种有效的体外诊断标志物。我们的微孔酶联免疫吸附试验比显微镜和其他商业ELISA更准确,比需要2周才能完成的“黄金标准”培养/酵母菌更快。我们现在已经开发出抗原亲和纯化抗体,使我们能够将测试的灵敏度提高十倍。我们建议将改进后的抗体用于试纸,可用于现场快速检测粪便抗原。为了使只检测固定标本的实验室(阿米巴病的大多数误诊发生在美国)可以使用免疫分析,我们将开发检测福尔马林稳定的内阿米巴抗原的免疫分析方法。我们还建议开发一种基于抗体的内阿米巴包囊检测方法。将开发针对包囊的多克隆和单克隆抗体,并将其用于对受污染的水和食物进行免疫分析(弗吉尼亚州莱斯顿和乔治亚共和国第布里西最近分别爆发了食物和水传播疫情,这使这成为一个局部和紧迫的需要)。包囊特异性测试的开发将仿效贾第虫和隐孢子虫的包囊和卵囊特异性抗原的开发。收集的数据将用于开发510(K)文件,以提交给FDA并由其审查。该项目将导致一系列在阿米巴病诊断中有广泛应用的测试。这些测试将是评估我们独立的热带病研究单位开发的阿米巴病候选疫苗的关键工具。拟议的商业应用:将为阿米巴病开发新的诊断测试,以解决美国社区和参考实验室的误诊问题,实现对寄生虫的供水筛选,并允许对疫苗试验进行现场评估。我们的结果应该会导致商业测试,这将对世界各地的健康行业具有重要价值。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

David M. Lyerly其他文献

Cloning and expression of the toxin B gene ofClostridium difficile
  • DOI:
    10.1007/bf02095867
  • 发表时间:
    1990-06-01
  • 期刊:
  • 影响因子:
    2.600
  • 作者:
    John L. Johnson;Carol Phelps;Lisa Barroso;Mary D. Roberts;David M. Lyerly;Tracy D. Wilkins
  • 通讯作者:
    Tracy D. Wilkins
Mo1287 Older Hospitalized Patients With Ribotype 027 <em>Clostridium difficile</em> Infection and Stool Toxin Have More Intestinal Inflammation and an Increased Risk of Death
  • DOI:
    10.1016/s0016-5085(13)62320-3
  • 发表时间:
    2013-05-01
  • 期刊:
  • 影响因子:
  • 作者:
    James H. Boone;Laurie Archbald-Pannone;Robert J. Carman;Christine McCoy;Kimberly N. Wickham;Rachel Albert;Richard Guerrant;Chris Franck;David M. Lyerly
  • 通讯作者:
    David M. Lyerly
Nonspecific binding of mouse monoclonal antibodies toClostridium difficile toxins A and B
  • DOI:
    10.1007/bf01570105
  • 发表时间:
    1989-11-01
  • 期刊:
  • 影响因子:
    2.600
  • 作者:
    David M. Lyerly;Pauline E. Carrig;Tracy D. Wilkins
  • 通讯作者:
    Tracy D. Wilkins

David M. Lyerly的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('David M. Lyerly', 18)}}的其他基金

Alum-absorbed subunit vaccine to prevent intestinal amebiasis
预防肠道阿米巴病的明矾吸收亚单位疫苗
  • 批准号:
    8122722
  • 财政年份:
    2011
  • 资助金额:
    $ 29.76万
  • 项目类别:
Alum-absorbed subunit vaccine to prevent intestinal amebiasis
预防肠道阿米巴病的明矾吸收亚单位疫苗
  • 批准号:
    8303054
  • 财政年份:
    2011
  • 资助金额:
    $ 29.76万
  • 项目类别:
New Diagnostics for Amebiasis
阿米巴病的新诊断方法
  • 批准号:
    6736678
  • 财政年份:
    2004
  • 资助金额:
    $ 29.76万
  • 项目类别:
New Diagnostics for Amebiasis
阿米巴病的新诊断方法
  • 批准号:
    6895799
  • 财政年份:
    2004
  • 资助金额:
    $ 29.76万
  • 项目类别:
NEW APPROACHES TO THE DIAGNOSIS OF AMEBIASIS
阿米巴病诊断新方法
  • 批准号:
    6212204
  • 财政年份:
    2000
  • 资助金额:
    $ 29.76万
  • 项目类别:
Lectin Derived Peptides as an Anti-Adherence Vaccine for Amebiasis
凝集素衍生肽作为阿米巴病的抗粘附疫苗
  • 批准号:
    6324593
  • 财政年份:
    2000
  • 资助金额:
    $ 29.76万
  • 项目类别:
Lectin Derived Peptides as an Anti-Adherence Vaccine for Amebiasis
凝集素衍生肽作为阿米巴病的抗粘附疫苗
  • 批准号:
    6213068
  • 财政年份:
    1999
  • 资助金额:
    $ 29.76万
  • 项目类别:
SERODIAGNOSIS AND IMMUNOPROPHYLAXIS OF AMEBIASIS
阿米巴病的血清学诊断和免疫预防
  • 批准号:
    2871524
  • 财政年份:
    1995
  • 资助金额:
    $ 29.76万
  • 项目类别:
SERODIAGNOSIS AND IMMUNOPROPHYLAXIS OF AMEBIASIS
阿米巴病的血清学诊断和免疫预防
  • 批准号:
    2072937
  • 财政年份:
    1995
  • 资助金额:
    $ 29.76万
  • 项目类别:
SERODIAGNOSIS AND IMMUNOPROPHYLAXIS OF AMEBIASIS
阿米巴病的血清学诊断和免疫预防
  • 批准号:
    2538212
  • 财政年份:
    1995
  • 资助金额:
    $ 29.76万
  • 项目类别:

相似海外基金

Establishment and evaluation of a rapid diagnosis using nanotechnology for amebiasis
利用纳米技术对阿米巴病进行快速诊断的建立和评估
  • 批准号:
    17K08811
  • 财政年份:
    2017
  • 资助金额:
    $ 29.76万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Host-directed Therapeutics for Amebiasis
阿米巴病的宿主导向治疗
  • 批准号:
    9247887
  • 财政年份:
    2016
  • 资助金额:
    $ 29.76万
  • 项目类别:
Elucidation of the function of group 2 innate lymphoid cells in chronic and severe cases of amebiasis
阐明第 2 组先天淋巴细胞在慢性和严重阿米巴病病例中的功能
  • 批准号:
    16K19118
  • 财政年份:
    2016
  • 资助金额:
    $ 29.76万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Nanoformulation of Synergistic TLR Ligands to Enhance Amebiasis Vaccine Efficacy
协同 TLR 配体纳米制剂增强阿米巴病疫苗功效
  • 批准号:
    8769515
  • 财政年份:
    2014
  • 资助金额:
    $ 29.76万
  • 项目类别:
Immunological cohort study on amebiasis in South Asia
南亚阿米巴病免疫学队列研究
  • 批准号:
    26305013
  • 财政年份:
    2014
  • 资助金额:
    $ 29.76万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of a rapid diagnostic test for amebiasis by using fluorescent nanoparticles
使用荧光纳米粒子开发阿米巴病快速诊断测试
  • 批准号:
    26460516
  • 财政年份:
    2014
  • 资助金额:
    $ 29.76万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Planning Grant for the Efficacy of Auranofin for the Treatment of Amebiasis
金诺芬治疗阿米巴病功效的规划拨款
  • 批准号:
    8264457
  • 财政年份:
    2012
  • 资助金额:
    $ 29.76万
  • 项目类别:
The Role of Leptin in Susceptibility to Amebiasis
瘦素在阿米巴病易感性中的作用
  • 批准号:
    8473656
  • 财政年份:
    2012
  • 资助金额:
    $ 29.76万
  • 项目类别:
The Role of Leptin in Susceptibility to Amebiasis
瘦素在阿米巴病易感性中的作用
  • 批准号:
    8664787
  • 财政年份:
    2012
  • 资助金额:
    $ 29.76万
  • 项目类别:
The Role of Leptin in Susceptibility to Amebiasis
瘦素在阿米巴病易感性中的作用
  • 批准号:
    8057481
  • 财政年份:
    2012
  • 资助金额:
    $ 29.76万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了