Automated Monitoring of Chemotactic Cell Movement

描述（由申请人提供）：ECIS/出租车技术可以检测 细胞通过琼脂糖基质移动到小电极， 电阻，它们赋予电路，因为它们覆盖部分的 小电极该系统适用于细胞的快速通量分析。 在趋化因子和其他刺激细胞增殖的因子存在下， 运动这项技术具有重要的优势， 传统的细胞运动测量方法。首先，它能够评估细胞 对刺激的反应，而没有预先建立一个特定的终点。 第二，ECIS/出租车环境更紧密地反映了自然生物学 环境第三，数据可以根据以下方面进行定量评估： 电路电阻的变化。我们在第一阶段的结果是， 项目清楚地表明，这项技术可以用来测量 细胞对趋化因子的反应和药物对细胞的作用 趋化反应在第二阶段，我们会发展 可用于评估趋化性和趋化动力学的方案 哺乳动物白细胞的反应，以及原型的评价方法 化学运动的拮抗剂。 拟定商业应用： ECIS/taxis技术将立即用于寻找抗趋化性 试剂（抗炎剂），趋化剂（新趋化因子）， 已知的趋化因子（促炎剂）和运动刺激剂（促炎剂 代理商）。

DESCRIPTION ( provided by applicant): The ECIS/taxis technology can detect cells that move to a small electrode through an agarose matrix by the resistance that they impart to an electrical circuit as they cover parts of the small electrode. This system is amenable to rapid throughput analysis of cell behavior in the presence of chemokines and other agents that motivate cell movement. This technology has important advantages as an alternative to traditional measures of cell movement. First, it enables the evaluation of cell responses to stimuli without the preestablishment of a specific endpoint. Second, the ECIS/taxis environment more closely reflects the natural biological environment. Third, the data can be quantitatively assessed according to changes in the resistance of the circuit. Our result in the Phase I of this project clearly demonstrate that this technology can be used to measure the response of cells to chemotactic factors and effects of drugs on the chemotactic response. In Phase II of this project, we will develop the protocols that can be used to evaluate the chemotactic and chemokinetic responses of mammalian leukocytes, and the methods of evaluation of prototype antagonists of chemokinesis. PROPOSED COMMERCIAL APPLICATION: The ECIS/taxis technology will be immediately useful in the search for anti-chemotactic agent (anti-inflammatory agents), chemotactic agents (new chemokines), agonists of known chemokines (pro-inflammatory agents), and stimulators of motility (pro-inflammatory agents).