Developing Next-Generation Sequencing multiplexes for Birds of Prey: A pilot study for non-human forensics

为猛禽开发下一代多重测序：非人类法医学试点研究

• 批准号: 1791037
• 金额: --
• 依托单位: University of Leicester
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1791037
• 关键词: Developing; Next; Generation; Sequencing; multiplexes

Most forensic activity focuses on human DNA however analysis of animal and plant DNA can also contribute by linking locations, or providing species- or individual-level identification, for example in the context of wildlife- and eco-crime. Since the 1990s, individual-level analyses have been based on length differences at STR loci as ascertained by capillary electrophoresis, whilst traditional Sanger sequencing of mtDNA has determined ancestry and species-level identification. However over the last decade, massively-parallel sequencing (MPS) has revolutionised biology allowing cost-effective characterisation of whole genomes; this holds great promise as the next innovation in forensic analysis too. Illumina has developed a human forensic MPS kit that allows simultaneous testing of multiple autosomal and Y-specific STRs, mtDNA and SNPs. This multi-target approach conserves biological material whilst maximizing discrimination and efficiency. This same approach could be applied to animal and plant forensic evidence allowing both human and non-human tests to be performed using a common platform, bioinformatics pipeline, and ultimately reporting procedure. This studentship will explore the approach as applied to birds of prey (raptors). Illegal poisoning remains a threat to these birds and their theft from the wild for resale to the falconry trade is a lucrative business.

大多数法医活动集中在人类DNA上，但是对动物和植物DNA的分析也可以通过联系地点或提供物种或个人层面的鉴定（例如在野生动物和生态犯罪的背景下）来作出贡献。自20世纪90年代以来，个体水平的分析已经基于毛细管电泳确定的STR位点的长度差异，而传统的mtDNA Sanger测序已经确定了祖先和物种水平的鉴定。然而，在过去的十年中，大规模平行测序（MPS）已经彻底改变了生物学，允许具有成本效益的全基因组表征；这也为法医分析的下一个创新带来了巨大的希望。Illumina开发了一种人类法医MPS试剂盒，可以同时检测多个常染色体和y染色体特异性str、mtDNA和snp。这种多靶点方法在保护生物材料的同时最大限度地提高了识别和效率。同样的方法可以应用于动植物法医证据，允许使用通用平台、生物信息学管道和最终报告程序进行人类和非人类测试。本课程将探讨应用于猛禽（猛禽）的方法。非法下毒对这些鸟来说仍然是一个威胁，从野外偷走它们再卖给猎鹰业是一项有利可图的生意。