ALPHA-1,3-GALACTOSYLTRANSFERASE DEFICIENT PIGS

描述：(从申请者的描述中扫描)：改进 器官移植后免疫排斥反应的临床处理 因此，对捐赠器官的需求稳步增加。不幸的是， 努力增加可供移植的人体器官供应 未能跟上需求的步伐，而且似乎不太可能这样做。相当可观 研究和开发工作一直致力于动物的用途 器官，特别是来自猪的器官，作为人类的创新替代品 器官。然而，需要克服一些异种移植的障碍。 在临床使用猪器官之前。供体猪的基因工程， 包括用基因靶向修饰的一代猪，将是 克服这些障碍所必需的。提出了一个第二阶段计划，以修改 用于异种移植的猪，使用同源重组创造 既有基因敲除，也有敲入。核移植技术，使用捐赠者 携带所需基因修饰的成纤维细胞系将被用于 生成想要的创始动物。 建议的商业应用：不可用

DESCRIPTION: (Scanned from the Applicant's Description): Improvements in clinical management of immune rejection following organ transplantation have resulted in a steadily increasing demand for donor organs. Unfortunately, efforts to increase the supply of human organs available for transplantation have failed to keep pace with demand and appear unlikely to do so. Considerable research and development efforts have been devoted toward the use of animal organs, specifically those from pigs, as an innovative alternative to human organs. However, a number of xenotransplantation barriers need to be overcome prior to clinical use of pig organs. Genetic engineering of donor pigs, including generation of pigs with gene targeted modifications, will be necessary to overcome these barriers. A Phase II program is proposed to modify pigs for use in xenotransplantation, using homologous recombination to create both genetic knock-outs and knock-ins. Nuclear transfer technology, using donor fibroblast lines carrying the desired genetic modifications, will be used to generate the desired founder animals. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE