OPTICAL SECTIONING THAT PRESERVES USEFUL DEPTH OF FIELD

描述：(逐字摘自申请人的摘要)剩余的一份 显微镜面临的挑战是一种有效和高效的成像方法 三维结构。现有的显微镜方法不能同时 提供高分辨率和高景深，也不提供z轴 位置信息，而无需获取连续的光学部分堆叠 关注度不同。 发展了一种新的光学/计算广视场显微镜方法。 在这个项目的第一阶段，它可以在焦点平面上成像结构 具有高分辨率，同时图像散焦结构具有 高景深。此方法还可以有效地提取和显示 焦平面周围结构的相对z轴位置信息 而不需要改变显微镜的焦点。与许多其他人不同 显微镜的最新发展、简单性和预期的成本节约 这种方法的使用将扩大用户的可访问性。提高的效率 光子的利用有望减少光漂白和光毒性。 该项目的第一阶段证明了可行性。第二阶段旨在使 图像质量和图像采集效率的进一步提高。 建议的商业应用：不可用

DESCRIPTION: (Verbatim from the Applicant's Abstract) One of the remaining challenges for microscopy is an effective and efficient method for imaging three dimensional structures. Existing microscopy methods cannot simultaneously provide both high resolution and high depth-of-field, nor provide z-axis position information without acquiring a serial stack of optical sections at different levels of focus. A novel optical/computational wide-field microscopy method has been developed during Phase I of this project that can image structures in the plane of focus with high resolution, and at the same time image out of focus structures with high depth-of-field. This method also effectively extracts and displays the relative z-axis position information for structure around the plane of focus without having to change the focus of the microscope. In contrast to many other recent developments in microscopy, the simplicity and anticipated cost savings of this method will expand user accessibility. The increased efficiency in photon utilization is anticipated to decrease photobleaching and phototoxicity. Phase I of this project demonstrated feasibility. Phase II seeks to make further improvements in image quality and in efficiency of image acquisition. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE