CORE--TISSUE CULTURE

培养的细胞（主要但不限于内皮细胞）是 这四个项目都需要这个项目。核心将 主要服务于三个罗切斯特项目的要求，虽然 将有一些正在进行的技术转让，在两个方向之间， 核心和哈姆纳博士的项目 我们预计，通过结合大量 对培养系统的常规技术支持， 以实现成本和工作量的显著节省。然而，少校 这一核心设施的理由在于我们的经验， 目前项目期间，对于内皮细胞， 以及不同细胞类型（HUVEC， HMEC-1，BAEC）在各种生长构型-微通道中， 显微玻片、平行板系统等。

Cultured cells (primarily but not exclusively endothelial cells) are required by all four projects of this program project. This core will primarily serve the requirement of the three Rochester projects, although there will be some ongoing technology transfer in both directions between the core and Dr. Hamner's project. We anticipate that by combining much of the routine technical support for the culture systems we will be able to effect a significant saving in cost and effort. However, the major rationale for this core facility resides in our experience from the present project period that, for endothelial cells, there are stringent and differing requirements for growth the different ell types (HUVECs, HMEC-1s, BAECs) in the various growth configurations-microchannels, microslides, parallel plate systems etc.