A Novel and Non-Invasive Method of Dermal Sampling

一种新颖的非侵入性皮肤采样方法

• 批准号: 6436289
• 负责人: Samir S Mitragotri
• 金额: $ 7.13万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA BARBARA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2004-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6436289
• 关键词: analytical chemistry; cytotoxicity; environmental exposure; hazardous substances; histology; method development; necrosis; noninvasive diagnosis; occupational hazard; physical chemical interaction; sample collection; skin absorption; skin disorder diagnosis; skin irritation /irritant; surfactant; toxicology; ultrasonography

DESCRIPTION (provided by applicant): The general objective of the proposed studies is to develop a "novel method of collecting contaminants from the skin to assess cutaneous exposure to hazardous materials." This method utilizes low-frequency ultrasound in combination with surfactants to extract contaminants from the superficial as well as deeper layers of skin in a controlled manner. Contaminants extracted in this manner will be assessed using analytical methods to assess the exposure of the individual to hazardous substances. Due to its large size, skin offers one of the most vulnerable organs for exposure to contaminants. One of the major issues in assessing skin exposure is the lack of methods to collect samples in a controlled way. Accordingly, better methods of meaningful sample collection are desired. In the proposed studies, we plan to address this challenge. We hypothesize that a short application of low-frequency ultrasound along with surfactants can be used to extract contaminants from a fixed area of skin painlessly and non-invasively. The extracted sample can then be analyzed to assess dermal exposure to contaminants. The specific objectives of the proposed studies are as follows: i) Assess the Composition of Ultrasonically Collected Sample, ii) Assess Mechanisms of Ultrasonic Sample Collection, and iii) Assess Safety Issues of Ultrasound Application.

描述(由申请人提供)：拟议研究的总体目标是开发一种“从皮肤收集污染物以评估皮肤暴露于危险材料的新方法”。这种方法利用低频超声波和表面活性剂相结合，以受控的方式从皮肤表层和深层提取污染物。以这种方式提取的污染物将使用以下方法进行评估 评估个人接触危险物质的分析方法。 由于它的大小，皮肤是暴露在污染物中最脆弱的器官之一。评估皮肤暴露的主要问题之一是缺乏以受控方式收集样本的方法。因此，需要更好的有意义的样本收集方法。在拟议的研究中，我们计划应对这一挑战。 我们假设，短期应用低频超声波和表面活性剂可以无痛、无创地从皮肤的固定区域提取污染物。然后可以对提取的样本进行分析，以评估皮肤接触污染物的情况。拟议研究的具体目标如下：i)评估超声采集样本的组成；ii)评估超声波样本收集的机制；以及iii)评估超声波应用的安全问题。