Light regulation of gene expression and inter-tissue signalling

基因表达和组织间信号传导的光调节

• 批准号: 1801025
• 金额: --
• 依托单位: University of Sheffield
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1801025
• 关键词: Light; regulation; gene; expression; inter

Gene regulation is at the core of many biological processes. This project aims to examine how light signals regulate genes at the tissue level to determine whole organism responses in plants. Using the model organism Arabidopsis thaliana and specific transgenic lines developed in the Casson laboratory, this project will use next generation sequencing and bioinformatics analysis to interrogate light-dependent gene regulation at the tissue specific level. The project will examine both existing gene expression data and generate novel data sets for analysis with the aim of identifying tissue specific regulatory networks. A combination of molecular techniques including quantitative RT-PCR, reporter gene assays, Western blotting and mutant analysis will then examine the role of components of these networks in regulating light responses.

基因调控是许多生物过程的核心。该项目旨在研究光信号如何在组织水平上调节基因，以确定植物中整个生物体的反应。利用模式生物拟南芥和卡森实验室开发的特定转基因品系，该项目将使用下一代测序和生物信息学分析来询问组织特异性水平上的光依赖性基因调控。该项目将检查现有的基因表达数据，并生成新的数据集进行分析，目的是确定组织特异性调控网络。分子技术的组合，包括定量RT-PCR，报告基因测定，蛋白质印迹和突变分析，然后将检查这些网络的组件在调节光反应的作用。