DNA repair and mutagenesis at replication forks: analysis of HelQ and Rrm3 helicases

复制叉处的 DNA 修复和诱变：HelQ 和 Rrm3 解旋酶的分析

• 批准号: 1803154
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1803154
• 关键词: DNA; repair; mutagenesis; replication; forks

The project focuses on HelQ, a human DNA repair enzyme. Malfunctions in HelQ are associated with the onset of some cancers, by mechanisms that are unclear but seem to relate to the ability of HelQ to interact with Rad51 and Rad51 paralogue enzymes. The rotation will spend a few weeks doing biochemical analysis of helicase enzymes by (a) the effects of site-directed mutagenesis on ATPase, DNA binding and helicase activity and/or (b) analysis of helicase protein-protein interactions with Rad51 and its paralogues.

该项目的重点是HelQ，一种人类DNA修复酶。HelQ中的功能障碍与某些癌症的发病有关，其机制尚不清楚，但似乎与HelQ与Rad 51和Rad 51 parabolic酶相互作用的能力有关。该轮将花费几周时间对解旋酶进行生化分析，方法是（a）定点诱变对ATP酶、DNA结合和解旋酶活性的影响和/或（B）分析解旋酶蛋白与Rad 51及其旁系同源物的相互作用。