GENETIC MAPPING OF MOUSE CHROMOSOMAL GENES

小鼠已被用作基因组分析的模型哺乳动物系统。 我们一直致力于通过分析来自种间或亚种间交配的几个多位点杂交来开发小鼠的高密度遗传图谱。 来自这些小鼠的DNA已被分型为1200多个位点，其中约一半也被其他研究人员绘制了遗传图谱。 这使我们能够将新定义的基因映射到遗传图谱中的特定位置，并将我们的数据整合到每条染色体的合成图谱中。 重点放在绘制表达的基因，允许与其他物种的地图进行比较分析，并有助于努力确定遗传性发育障碍的基因。 最近，这些研究导致了编码新的同源异型盒基因、免疫系统基因、过氧化物酶基因和参与牙齿发育的基因的基因图谱。 我们最近也开始使用辐射杂交DNA面板开发一个综合的物理和遗传图谱的Chr 5。 到目前为止，我们已经添加了近50个位点的RH图的这条染色体。特定的地图位置可能出于几个原因而有用。 特别地，接近已知的发育突变可以将该基因鉴定为异常表型的潜在候选者。今年，我们使用消减杂交来鉴定干扰素产生调控的候选基因。 使用If1不同的小鼠进行消减杂交，以克隆在这些小鼠中差异表达的一系列基因。 基因被映射到小鼠基因组中的多个位点，包括一个新基因，该基因被映射到远端3号染色体，因此代表If1的候选基因。

The mouse has been used as the model mammalian system for genome analysis. We have been engaged in the development of a high density genetic map of the mouse by analysis of several multilocus crosses derived from interspecies or intersubspecies matings. DNAs from these mice have been typed for over 1200 loci, about half of which have also been genetically mapped by other investigators. This permits us to map newly defined genes to specific positions in the genetic map, and to integrate our data into composite maps of each chromosome. Emphasis has been placed on mapping expressed genes to permit comparative analysis with the maps of other species and to contribute to efforts to identify genes involved in inherited developmental disorders. These studies have, most recently, resulted in the genetic mapping of genes encoding new homeobox genes, immune system genes, peroxiredoxin genes and genes involved in tooth development. We have also recently begun using a radiation hybrid DNA panel to develop an integrated physical and genetic map of Chr 5. To date, we have added almost 50 loci to the RH map of this chromosome. Specific map locations can be useful for several reasons. In particular, proximity to a known developmental mutation can identify the gene as a potential candidate for the abnormal phenotype. This year we used subtractive hybridization to identify candidate genes for the regulation of interferon production. Subtractive hybridization using mice that differ at If1 was used to clone a series of genes differentially expressed in these mice. Genes were mapped to multiple sites in the mouse genome including one novel gene which was mapped to distal chromosome 3 and thus represents a candidate for If1.