Microehrology in DNA-hydrogels for Molecular imprinting

用于分子印迹的 DNA 水凝胶微生态学

• 批准号: 1805384
• 金额: --
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1805384
• 关键词: Microehrology; DNA; hydrogels; Molecular; imprinting

In the past few years DNA has been proven to be a material that allows building almost any nano-sized structures ranging from well-defined sheets, to wires and finite-sized 3D building blocks. In this project the student will use some of the well-established DNA-binding rules to build a new class of 3-dimensional DNA-hydrogels with well-defined structures. These involve the use of the fact that we can form 3-way and 4-way junctions with different strands of single-stranded (ss) DNA and use these then to build 3D structures. Aim is to build in freely dangling ssDNA that is still attached to the network for sensing unknown DNA fragments. Once these fragment bind the viscoelastic properties of the hydrogel will change significantly. In order to test these changes, the student will build up a micro-rheometer based on optically trapping a probe particle, whose fluctuations will be used to extract the viscoelastic properties of the network. We will employ a home-developed algorithm for that [2].Seeman, N. C. Nature 421, 427 (2003); Rothemund, P. W. K. Nature 440, 297 (2006) ; Kershner, R. J. et al. Nature Nanotechnology 4, 557 (2009); Ke, J. et al. Nature Chemistry 6, 994 (2014)[2] T. Yanagishima, D. Frenkel, J. Kotar, and E. Eiser, "Real-time monitoring of complex moduli from micro-rheology", J.Phys.: Condens. Matter, 23, 194118 (2011)

在过去的几年中，DNA已被证明是一种可以构建几乎任何纳米尺寸结构的材料，从定义明确的薄片到电线和有限尺寸的3D构建块。在这个项目中，学生将使用一些已经建立的DNA结合规则来构建一类具有明确结构的新型三维DNA水凝胶。这些涉及利用这样一个事实：我们可以与不同链的单链（ss）DNA形成3向和4向连接，然后使用这些连接来构建3D结构。目标是构建仍然附着在网络上的自由悬挂的ssDNA，以检测未知的DNA片段。一旦这些片段结合，水凝胶的粘弹性质将显著改变。为了测试这些变化，学生将建立一个基于光学捕获探针粒子的微流变仪，其波动将用于提取网络的粘弹性。我们将采用一种自行开发的算法[2]。C. Nature 421，427（2003）; Rothemund，P. W. K. Nature 440，297（2006）; Kershner，R.等人Nature Nanotechnology 4，557（2009）; Ke，J.等人Nature Chemistry 6，994（2014）[2] T. Yanagishima，D. Frenkel，J. Kotar，and E. Eiser，“从微观流变学实时监测复合模量”，J.Phys.：康登斯Matter，23，194118（2011）

项目成果

Bulk rheology of sticky DNA-functionalized emulsions.

• DOI: 10.1103/physreve.104.054602
• 发表时间: 2021-11
• 期刊: Physical review. E
• 作者: I. Stoev;A. Caciagli;Anasua Mukhopadhyay;C. Ness;E. Eiser

Sprouting Droplets Driven by Physical Effects Alone.

仅由物理效应驱动的水滴萌芽。

• DOI: 10.1021/acs.langmuir.6b04514
• 发表时间: 2017
• 期刊: the ACS journal of surfaces and colloids
• 作者: Rumble KA

Discotic liquid crystals of cucurbit[7]uril (CB[7])-functionalised Laponite clays

• DOI: 10.1080/00268976.2018.1508778
• 发表时间: 2018-08
• 期刊: Molecular Physics
• 影响因子: 1.7
• 作者: P. Xu;Y. Lan;Li-Jun Dai;T. O’Neill;I. Stoev;Tianyang Cao;E. Eiser

Microrheology of DNA hydrogels.

DNA水凝胶的微观流变学

• DOI: 10.1073/pnas.1722206115
• 发表时间: 2018-08-07
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Xing Z;Caciagli A;Cao T;Stoev I;Zupkauskas M;O'Neill T;Wenzel T;Lamboll R;Liu D;Eiser E
• 通讯作者: Eiser E

Using single-beam optical tweezers for the passive microrheology of complex fluids

• DOI: 10.1117/12.2318019
• 发表时间: 2018-09
• 作者: I. Stoev;A. Caciagli;Zhongyang Xing;E. Eiser