Compact Tunable Mid-IR/UV Laser/Ion source for IR MALDI

用于 IR MALDI 的紧凑型可调谐中红外/紫外激光/离子源

• 批准号: 6529866
• 负责人: RHETT JAMES BARNES
• 金额: $ 22.65万
• 依托单位: OPOTEK, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2004-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6529866
• 关键词: biomedical equipment development; energy source; evaluation /testing; infrared spectrometry; lasers; matrix assisted laser desorption ionization; optics

The goal of this Phase II STTR cooperative research program between OPOTEK and Emory University is to continue development of a small and inexpensive infrared laser based on optical parametric oscillator (OPO) technology and to develop a target system specifically designed for liquid matrix IR-MALDI. This package of IR laser and ion source is designed for high-end MALDI mass spectrometers, and will allow the user to switch rapidly between IR and UV MALDI modes. The research is divided into two sections: development of the IR/UV laser based on our Phase I laser system; and development of and ion source for IR- MALDI, tailored to the experimental conditions that were found to be ideal for IR-MALDI from liquid matrices. Open areas for commercial analytical applications where IR MALDI has advantages over UV MALDI include analysis of high-mass DNA, the analysis of other high molecular weight biomolecules (100 - 500 kDa), analysis of biopolymers from gel blots or directly from cell or tissue samples, and the analysis of pharmaceuticals and other low molecular weight molecules (200 - 1500 Da). Work will be performed to try to optimize our laserlon-source package for performance in these areas. PROPOSED COMMERCIAL APPLICATIONS: The need for small and inexpensive IR light sources for matrix assisted laser desorption ionization (MALDI) mass spectrometry is great. Commercial applications for the compact IR OPO either as a stand alone retrofit unit or as an option for new mass spectrometers include analysis of high mass DNA and proteins, gel separated proteins, low molecular weight species, direct liquid introduction and frozen tissue sections.

Opotek和Emory University之间的II阶段STTR合作研究计划的目标是，基于光学参数振荡器（OPO）技术，继续开发小型且廉价的红外激光器，并开发专门为液体矩阵IRMALDI设计的目标系统。此IR激光和离子源的包装是为高端MALDI质谱仪设计的，它将允许用户在IR和UV MALDI模式之间迅速切换。该研究分为两个部分：根据我们的I阶段激光系统开发IR/UV激光器；以及针对实验条件量身定制的IR MALDI的离子和离子源的发展，这些条件是液体矩阵中IR-Maldi的理想选择。 IR MALDI比紫外线MALDI具有优势的商业分析应用领域包括对高质量DNA的分析，对其他高分子重量生物分子（100-500 kDa）的分析，分析来自凝胶印迹的生物聚合物或直接来自细胞或组织样品的生物聚合物，以及对药物的分析以及其他低分子量和其他低分子量和其他低分子量摩尔西尔·摩尔摩尔摩尔菌（200）将进行工作以尝试优化我们的Laserlon-Source软件包，以在这些领域的性能。拟议的商业应用：对基质辅助激光电离电离（MALDI）质谱的小且廉价的红外光源的需求非常好。紧凑型IR OPO的商业应用要么是独立的翻新单元，要么是新的质谱仪的一种选择，包括对高质量DNA和蛋白质的分析，凝胶分离的蛋白质，低分子量物种，直接液体引入和冷冻组织切片。