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Ultrafast 2DIR Spectroscopy for Biomedical Analysis

用于生物医学分析的超快 2DIR 光谱

基本信息

批准号：
1810876
负责人：
金额：
--
依托单位：
University of Strathclyde
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2016
资助国家：
英国
起止时间：
2016 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=studentship-1810876
关键词：
Ultrafast 2DIR Spectroscopy Biomedical Analysis

项目摘要

The project explores the application of ultrafast 2D-IR spectroscopy as a novel analytical tool for use in biomedical analysis. 2D-IR spectroscopy has been demonstrated to be a powerful tool for use in analysing complex spectra and for obtaining information on molecular structure, spectroscopy and dynamics of a range of biological molecules. This work has paved the way for the method to be applied in the analysis of complex biofluids such as blood plasma or serum for rapid, non-invasive disease diagnosis. However, significant technical and physical science challenges must be overcome in order to make the measurements practical and to develop the tools required for a comprehensive understanding of the spectra produced.This project will begin by obtaining 2D-IR spectra of model blood plasma consisting of aqueous solutions of soluble, commercially-available proteins, making the mixtures more complex as the project progresses in order to establish measurement protocols and the limits of experimental sensitivity. To put the challenges into context, these will be the first measurements, on a global scale examining the amide I 2D-IR response of proteins in aqueous solution, which will offer considerable new insight into the dynamics of proteins in solution and the impact of the often-employed deuteration IR spectroscopy technique on our current understanding of their molecular physics. In parallel with this work will be development of data analysis tools based on multivariate and higher order analysis for data presentation and information retrieval.

该项目探讨了超快2D-IR光谱作为一种新型分析工具在生物医学分析中的应用。二维红外光谱已被证明是一个强大的工具，用于分析复杂的光谱和获得信息的分子结构，光谱和动力学的一系列生物分子。这项工作为该方法应用于复杂生物流体（如血浆或血清）的分析铺平了道路，以实现快速，非侵入性的疾病诊断。然而，为了使测量切实可行，并开发全面了解所产生的光谱所需的工具，必须克服重大的技术和物理科学挑战。该项目将开始，获得由可溶性、市售蛋白质的水溶液组成的模型血浆的二维红外光谱，随着项目的进展，使混合物更加复杂，以便建立测量协议和实验灵敏度的限制。为了将这些挑战置于背景下，这些将是第一次测量，在全球范围内检查酰胺I 2D-IR响应的蛋白质在水溶液中，这将提供相当多的新的见解蛋白质在溶液中的动力学和经常采用的氘代红外光谱技术的影响，我们目前的理解他们的分子物理。与此同时，将开发基于多元和高阶分析的数据分析工具，用于数据呈现和信息检索。