BD Biosciences LSR Flow Cytometer

BD Biosciences LSR 流式细胞仪

• 批准号: 6440371
• 负责人: RICHARD R HARDY
• 金额: $ 18.1万
• 依托单位: RESEARCH INST OF FOX CHASE CAN CTR
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440371
• 关键词: biomedical equipment; biomedical equipment development; cytology; flow cytometry

DESCRIPTION (provided by applicant): A group of nine NIH-funded major users and four secondary NIH-funded users at Fox Chase Cancer Center request funds for a BD Biosciences LSR flow cytometer. The currently available instrumentation that is capable of providing >3 color analysis, a BD FACStar' plus cell sorter is at capacity, particularly due to increasing demands for sorting. The other instrument, a BD FACScan, is widely used for simple immunophenotyping (2 color, P1) and for cell cycle analysis, but cannot perform more complex multi-color analyses that are increasingly required by use of various fluorescent reporter systems (e.g., IRES-GFP retroviruses). Furthermore, many of the users in this application propose experiments that currently cannot be done with the present instrumentation, specifically determination of intracellular calcium levels or DNA content together with multicolor cell surface markers. The proposed instrument is a three-laser analyzer that will be user-operated, providing for maximum flexibility in scheduling and providing increased multicolor analysis capacity together with these new capabilities. While the research programs of the different users are quite diverse, including immunobiology, virology, and cell biology, they all have as an ultimate goal the understanding of human disease processes. This is being accomplished by studies of the mechanisms of lymphocyte development, imrnunoglobulin rearrangement, generation of functional subpopulations of effector cells (such as memory B cells, helper T cells, cytotoxic T cells, and NK cells), regulation of the cell cycle, signal transduction, and regulation of cell morphology. Some of the proposed experiments analyze viruses that infect the central nervous system. Others seek to increase our understanding how immune responses, both humoral and cellular, can be regulated, with implications for vaccination and also autoimmunity. Still others have a more clinical focus, seeking to develop a vaccine therapy for certain cancers. All of these projects have a common need for making multiple precise measurements of individual cells, typically in complex mixtures, a requirement that can be met effectively by the proposed instrument. The requested instrument will be housed and maintained in the Flow Cytometry Facility at FCCC. Users will be trained in its operation by Facility personnel. This shared Facility is an ideal location for this system as it provides Flow Cytometry technical expertise and instrument access to NIH funded laboratories at FCCC and is a hub of collaborative efforts with outstanding, highly trained technical support.

描述(由申请人提供)：由美国国立卫生研究院资助的九个主要用户和 福克斯·蔡斯癌症中心的四名由NIH资助的二级用户申请资金 BD Biosciences LSR流式细胞仪。当前可用的检测工具 能够提供&gt；3颜色分析，BD FACStar的PLUS细胞分选器位于 能力，特别是由于对分拣的需求不断增加。另一个 BD FACScan仪器广泛用于简单的免疫表型分析(2色， P1)和细胞周期分析，但不能执行更复杂的多色 越来越多地需要使用各种荧光报告程序进行分析 系统(例如，IRES-GFP逆转录病毒)。此外，这个网站中的许多用户 应用程序提出目前不能用目前进行的实验 仪器，特别是细胞内钙水平的测定或 DNA含量与多色细胞表面标记相结合。建议数 仪器是一个三激光分析器，将由用户操作，提供 最大限度地灵活安排日程并提供更多的多色分析 容量与这些新功能相结合。而美国的研究计划 不同的用户相当不同，包括免疫生物学、病毒学和 细胞生物学，他们都把理解人类作为一个终极目标 疾病过程。这是通过对其机制的研究来实现的 淋巴细胞发育，免疫球蛋白重排，功能性 效应细胞亚群(如记忆B细胞，辅助T细胞， 细胞毒性T细胞和NK细胞)，细胞周期的调节，信号 转导和细胞形态的调节。一些建议的 实验分析了感染中枢神经系统的病毒。其他人则在寻找 为了加深我们对体液和细胞免疫反应的理解， 可能受到监管，对疫苗接种和自身免疫也有影响。 还有一些人更注重临床，寻求开发一种疫苗疗法 治疗某些癌症。所有这些项目都有一个共同的需求，即 对单个细胞进行多次精确测量，通常是在复杂的情况下 混合物，这是拟议的文书可以有效满足的要求。 所要求的仪器将放置在流式细胞仪中并进行维护 在fccc的设施。设施人员将对用户进行操作培训。 该共享设施是该系统的理想位置，因为它提供流量 NIH资助的实验室的细胞学技术专长和仪器使用情况 是一个合作努力的中心，拥有优秀的、训练有素的 技术支持。