Muscle aging and gene expression balance in Caenorhabditis elegans

秀丽隐杆线虫的肌肉衰老和基因表达平衡

• 批准号: 1827305
• 金额: --
• 依托单位: University of Leeds
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1827305
• 关键词: Muscle; aging; gene; expression; balance

Genome editing based on CRISPR-Cas9 is now rapid and straightforward in Caenorhabditis elegans and has opened up entirely new avenues of research in this key model system. Precise manipulations of promoter regions in situ will allow subtle modification in vivo of expression levels of proteins with roles in control of muscle calcium ion homeostasis. Discordance in levels of subunits of the complex calcium ion channel regulatory system is predicted to lead to muscle ageing, the major factor in mobility decline in older people. A better understanding of muscle ageing in animals may lead to delaying of muscle ageing in people.

基于 CRISPR-Cas9 的基因组编辑现在在秀丽隐杆线虫中快速而简单，并为这一关键模型系统的研究开辟了全新的途径。原位启动子区域的精确操作将允许在体内微妙地改变在控制肌肉钙离子稳态中起作用的蛋白质的表达水平。复杂的钙离子通道调节系统亚基水平的不一致预计会导致肌肉衰老，这是老年人活动能力下降的主要因素。更好地了解动物的肌肉衰老可能会导致人类肌肉衰老的延迟。

项目成果

Complex motility and drug response phenotypes associated with myopathic variants in the ryanodine receptor.

与兰尼定受体肌病变异相关的复杂运动和药物反应表型。

• 发表时间: 2019
• 作者: Graham, B.L.

Short-term associative memory in Caenorhabditis elegans with subtle defects in the Ryanodine Receptor.

秀丽隐杆线虫的短期联想记忆，其瑞尼丁受体存在细微缺陷。

• 发表时间: 2018
• 作者: Graham B

Muscle Ageing and Gene Expression Balance in Caenorhabditis elegans.

秀丽隐杆线虫的肌肉衰老和基因表达平衡。

• 发表时间: 2016
• 作者: Graham B

The ryanodine receptor, lifespan and healthspan in the Caenorhabditis elegans model system

秀丽隐杆线虫模型系统中的兰尼碱受体、寿命和健康期

• 发表时间: 2016
• 作者: Graham B

Single Amino Acid Changes in the Ryanodine Receptor in the Human Population Have Effects In Vivo on Caenorhabditis elegans Neuro-Muscular Function

• DOI: 10.3389/fgene.2020.00037
• 发表时间: 2020-02-26
• 期刊: FRONTIERS IN GENETICS
• 影响因子: 3.7
• 作者: Graham, Brittany;Shaw, Marie-Anne;Hope, Ian A.
• 通讯作者: Hope, Ian A.