NEW RNA BINDING DOMAINS SELECTED BY RIBOSOME DISPLAY

描述(改编自申请者的描述)：本项目审查 利用核糖体展示方法学筛选新作的可行性 与新的RNA靶标特异结合的RNA结合域(RBD)。如果 如果成功，可能会有可能设计出可以调节 在无细胞系统和多种环境中的基因表达和DNA复制 转基因细胞和生物，并使用这些蛋白质来研究和 高度控制细胞或病毒控制过程 特异性和准确性。这反过来可以帮助开发新的 治疗应用。该项目的风险围绕着两个不确定性： 1)当前核糖体展示的翻译/选择方法可能不是 足以满足所提议的应用，以及2)一些RNA结合域可以 需要九个以上的氨基酰基残基才能确定靶标的特异性。 以人U1a Rbd1为起始模板的初步结果表明 这是可行的，但许多问题仍未解决，可能被证明是无法克服的。

DESCRIPTION (adapted from the applicant's description): This project examines the feasibility of using ribosome display methodology to select for novel RNA-binding domains (RBDs) that specifically bind to new RNA targets. If successful, it may become possible to engineer novel RBDs that can regulate gene expression and DNA replication in cell-free systems and in a wide variety of transgenic cells and organisms, and to use such proteins to study and manipulate cellular or viral control processes with a high degree of specificity and accuracy. This could in turn aid the development of new therapeutic applications. The project's risk revolves around two uncertainties: 1) the current translation/selection methods of ribosome display may not be adequate for the proposed application, and 2) some RNA-binding domains may require more than nine aminoacyl residues to determine target specificity. Initial results using the human U1A RBD1 as a starting template indicate feasibility, but many problems remained unsolved and may prove insurmountable.