NEW RNA BINDING DOMAINS SELECTED BY RIBOSOME DISPLAY

通过核糖体显示选择的新 RNA 结合域

• 批准号: 6387204
• 负责人: KATHLEEN B HALL
• 金额: $ 11.55万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6387204
• 关键词: RNA binding protein; binding sites; molecular cloning; peptide library; polymerase chain reaction; protein binding; protein sequence; ribosomes

DESCRIPTION (adapted from the applicant's description): This project examines the feasibility of using ribosome display methodology to select for novel RNA-binding domains (RBDs) that specifically bind to new RNA targets. If successful, it may become possible to engineer novel RBDs that can regulate gene expression and DNA replication in cell-free systems and in a wide variety of transgenic cells and organisms, and to use such proteins to study and manipulate cellular or viral control processes with a high degree of specificity and accuracy. This could in turn aid the development of new therapeutic applications. The project's risk revolves around two uncertainties: 1) the current translation/selection methods of ribosome display may not be adequate for the proposed application, and 2) some RNA-binding domains may require more than nine aminoacyl residues to determine target specificity. Initial results using the human U1A RBD1 as a starting template indicate feasibility, but many problems remained unsolved and may prove insurmountable.

描述（根据申请人的描述改编）：此项目检查 使用核糖体显示方法选择新颖的可行性 特异性结合新RNA靶标的RNA结合结构域（RBD）。如果 成功，可以设计可以调节的小型RBD 无细胞系统中的基因表达和DNA复制 转基因细胞和生物，并使用此类蛋白质研究和 高度操纵细胞或病毒控制过程 特异性和准确性。这反过来可能有助于发展 治疗应用。该项目的风险围绕两个不确定性旋转： 1）当前的核糖体显示的翻译/选择方法可能不是 足以适合提议的应用，2）一些RNA结合域可能 需要超过9个氨基酰基残基来确定目标特异性。 使用人U1A RBD1作为起始模板的初始结果表示 可行性，但许多问题仍未解决，可能无法克服。