Role of IL-1 in Yersinia Induced Intestinal Inflammation

小肠结肠炎耶尔森氏菌感染是一种食源性病原体，可引起严重的肠道炎症。最近克隆了一种调节剂rovA，它不会引起严重的肠道炎症。rovA突变体对耶尔森氏菌感染不诱导IL-1 α。该提案的目的是确定哪些细菌基因负责这种表型，以及确定细菌蛋白质相互作用的宿主因子。将研究IL-1在鼠模型中观察到的死亡率中的作用。具体目标将通过利用耶尔森氏菌和小鼠模型的遗传易处理性来解决。已经完成了rovA调节基因的筛选。将筛选这些基因在体内诱导IL-1的能力。将对炎症进行组织学评估。宿主因子将通过产生封闭抗体来鉴定

Yersinia enterocolitica infection is a food born pathogen that causes severe intestinal inflammation. Recently a regulator was cloned, rovA, that does not cause severe intestinal inflammation. The rovA mutant does not induce IL-1alpha in response to Yersinia infection. The aim of this proposal is to determine which bacterial genes are responsible for this phenotype as well as identifying the host factors that the bacterial proteins interact with. The role of IL-1 in the mortality seen in the murine model will be investigated. The specific aims will be addressed by taking advantage of the genetic tractability of yersinia and the murine model. A screen for rovA regulated genes has been done. These genes will be screened for their ability to induce IL-1 in vivo. Inflammation will be assessed histologically. The host factors will be identify by producing blocking antibodies