CORE--MOUSE COAGULALTION LABORATORY

核心--小鼠凝血实验室

• 批准号: 6504161
• 负责人: ALVIN H SCHMAIER
• 金额: $ 13.59万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6504161
• 关键词: animal colony; antithrombins; biomedical facility; blood chemistry; blood coagulation; blood donor; clinical research; coagulation factor V; coagulation factor X; fibrinolysis; fibrinopeptide; human subject; immunochemistry; laboratory mouse; phlebotomy; plasminogen activator; prothrombin; von Willebrand factor

The Mouse Coagulation Laboratory will adapt standard clinical coagulation and fibrinolysis assays developed for the analysis of human specimens to the study of mouse samples. Under the direction of Dr. Alvin Schmaier, the director of the University of Michigan Hospitals coagulation Laboratory, this core will provide a centralized resource for the performance by dedicated coagulation laboratory personnel of a series of assays standardized for experimental mice. The specific aims of the Mouse coagulation Laboratory Core are: Specific aim #1: Establish coagulation-based assays with mouse plasma (PT, APTT, thrombin time, fibrinogen, factor assays, APC resistance). Specific aim #2: Establish assays to assess the fibrinolytic system in mouse plasma (whole plasma fibrinolysis plate, tPa and PAI-1 activity and antigen, alpha 2antiplasmin, plasminogen activity and antigen). Specific aim #3: Apply or establish immunochemical assays to measure mouse von Willebrand factor, factor V, factor VIII, fibrinogen, tissue plasminogen activator, plasminogen activator inhibitor-1, thrombin-antithrombin III complexes, and fibrinopeptide A. Establish amidolytic assays for C1 inhibitor and antithrombin III. Specific aim #4: Establish immunochemical assays to measure mouse activation peptides of factor X and prothrombin (Prothrombin fragment 1+2). The services of the Mouse coagulation laboratory will be used by all of the individual projects contained in this PPG proposal. Significant gains in economy, efficiency and accuracy are anticipated to result from consolidation of these activities into one, centralized core laboratory.

小鼠凝血实验室将采用标准临床试验 凝血和纤维蛋白溶解试验，用于分析 人类样本的研究小鼠样本。 指导下 阿尔文·施迈尔博士，密歇根大学的校长， 医院凝血实验室，这一核心将提供一个集中的 专用凝血实验室的性能资源 一系列标准化实验小鼠试验的人员。 小鼠凝血实验室核心的具体目标是： 具体目标#1：建立小鼠血浆的基于凝固的测定 (PT、APTT、凝血酶时间、纤维蛋白原、因子测定、APC 电阻）。 具体目标#2：建立测定法以评估 小鼠血浆（全血浆纤溶板、tPa和派-1活性 和抗原，α 2抗纤溶酶，纤溶酶原活性和抗原）。 具体目标#3：应用或建立免疫化学测定法， 测量小鼠血管性血友病因子，因子V，因子VIII， 纤维蛋白原，组织型纤溶酶原激活剂，纤溶酶原激活剂 抑制剂-1、凝血酶-抗凝血酶III复合物和纤维蛋白肽 A. 建立C1抑制剂和抗凝血酶III的酰胺分解试验。 具体目标#4：建立免疫化学测定以测量小鼠 因子X和凝血酶原的活化肽（凝血酶原片段 1+2）。 小鼠凝血实验室的服务将被所有 本项目编制补助金提案中所载的各个项目。 显著 预计将在经济性、效率和准确性方面有所提高， 从将这些活动合并为一个集中的核心， 实验室