CORE--MOUSE COAGULALTION LABORATORY

小鼠凝血实验室将适应标准临床 凝血和纤溶试验用于分析 以人体标本为研究对象，以小鼠标本为研究对象。在指导下 密歇根大学校长阿尔文·施迈尔博士 医院凝血实验室，这一核心将提供一个集中的 专用凝血实验室的性能资源 人员对实验小鼠进行了一系列标准化检测。 老鼠凝血实验室核心的具体目标是： 具体目标1：建立基于凝血的小鼠血浆检测方法 (PT、APTT、凝血酶时间、纤维蛋白原、因子分析、APC 抵抗)。 具体目标#2：建立评估纤溶系统的检测方法 小鼠血浆(全血浆纤溶平板、TPA和PAI-1活性 和抗原、α2抗纤溶酶、纤溶酶原活性和抗原)。 具体目标3：应用或建立免疫化学检测以 测量老鼠的von Willebrand因子，因子V，因子VIII， 纤维蛋白原、组织纤溶酶原激活剂、纤溶酶原激活剂 抑制物-1、凝血酶-抗凝血酶III复合体和纤维蛋白肽 建立C1抑制物和抗凝血酶III的氨解分析方法。 具体目标#4：建立免疫化学方法来检测小鼠 凝血因子X和凝血酶原激活肽(凝血酶原片段 1 2)。 老鼠凝血实验室的服务将由所有人使用 这份PPG提案中所包含的个别项目。意义重大 预计将在经济性、效率和准确性方面取得进展 将这些活动整合为一个集中的核心 实验室。

The Mouse Coagulation Laboratory will adapt standard clinical coagulation and fibrinolysis assays developed for the analysis of human specimens to the study of mouse samples. Under the direction of Dr. Alvin Schmaier, the director of the University of Michigan Hospitals coagulation Laboratory, this core will provide a centralized resource for the performance by dedicated coagulation laboratory personnel of a series of assays standardized for experimental mice. The specific aims of the Mouse coagulation Laboratory Core are: Specific aim #1: Establish coagulation-based assays with mouse plasma (PT, APTT, thrombin time, fibrinogen, factor assays, APC resistance). Specific aim #2: Establish assays to assess the fibrinolytic system in mouse plasma (whole plasma fibrinolysis plate, tPa and PAI-1 activity and antigen, alpha 2antiplasmin, plasminogen activity and antigen). Specific aim #3: Apply or establish immunochemical assays to measure mouse von Willebrand factor, factor V, factor VIII, fibrinogen, tissue plasminogen activator, plasminogen activator inhibitor-1, thrombin-antithrombin III complexes, and fibrinopeptide A. Establish amidolytic assays for C1 inhibitor and antithrombin III. Specific aim #4: Establish immunochemical assays to measure mouse activation peptides of factor X and prothrombin (Prothrombin fragment 1+2). The services of the Mouse coagulation laboratory will be used by all of the individual projects contained in this PPG proposal. Significant gains in economy, efficiency and accuracy are anticipated to result from consolidation of these activities into one, centralized core laboratory.