POPULATION STRUCTURE OF SCHISTOSOMES AND HOST SNAILS

血吸虫和寄主钉螺的种群结构

• 批准号: 6497095
• 负责人: DENNIS J. MINCHELLA
• 金额: $ 21.94万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-02-01 至 2004-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6497095
• 关键词: Brazil; Mollusca; Schistosoma; alleles; animal population genetics; clinical research; epizootiology; feces analysis; gene expression; gene frequency; gene mutation; genetic mapping; genetic markers; genetic polymorphism; genetic strain; genotype; geographic site; helminth genetics; host organism interaction; human subject; laboratory mouse; molecular cloning; nucleic acid probes; parasitic disease chemotherapy; phenotype; restriction fragment length polymorphism

Interactions between schistosomes and their snail hosts play an important role in the development of the host and parasite genetic systems. The extent of genetic heterogeneity in local parasite populations has implications on the epidemiology of host disease. Schistosoma mansoni exhibits significant phenotypic differences within and among geographic populations, yet the extent and nature of genetic variability residing in this parasite and its host snails remains largely unknown. A combination of existing multi-locus DNA markers and forthcoming single locus molecular probes (microsatellites) will be used to quantify the degree of host and parasite genetic heterogeneity in natural populations; further, we will determine how that variation changes on a spatial and temporal scale. Specific aims include: 1) determination of how genetic variation is partitioned among the various parasite and snail host subpopulations at each transmission site; and 2) assessment of relative genetic structure within and between populations of both snail host and schistosomes, and quantitative comparison of temporal genetic changes in schistosomes following chemotherapy. Results will yield evolutionary insights into the epidemiological process, help identify genetic consequences of control strategies, and complement concurrent immuno-epidemiology studies of humans in these Brazilian communities.

血吸虫和它们的蜗牛宿主之间的相互作用起着重要的作用