HIV INDUCED CELLULAR PATHOLOGY

HIV 引起的细胞病理学

• 批准号: 6475498
• 负责人: DAVID BALTIMORE
• 金额: $ 28.56万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6475498
• 关键词: MHC class I antigen; apoptosis; cellular pathology; cytotoxic T lymphocyte; flow cytometry; human immunodeficiency virus 1; regulatory gene; tissue /cell culture; transcription factor; virus infection mechanism

DESCRIPTION: The goal of this proposal is to understand the nature of transcription factor binding, apoptosis and the role of Nef and Nef-binding proteins in the avoidance of killing by MHC class I-restricted CTLs. The role of Nef in down regulating MHC class I expression will be assessed using techniques that allow characterization by flow cytometric analysis of events in primary and cultured cells with high-titered mutant or wild-type viruses.

描述：本提案的目标是了解 转录因子结合、凋亡及Nef和Nef结合的作用 蛋白质在避免由MHC I类限制性CTL杀伤中的作用。 的 Nef在下调I类MHC表达中的作用将通过使用 允许通过事件的流式细胞术分析表征的技术 在原代和培养细胞中用高滴度突变体或野生型病毒。