Understanding the role of secretion in phosphate uptake in plants

我们的动机是促进作物的发展，更有效地吸收磷酸盐(P)，一种必需的宏量营养素，并减少对有限的磷肥料的依赖。我们打算确定蛋白质分泌和根膜运输的机制，这是植物根中磷酸盐动员差异的基础。磷是所有生物体必需的大量营养素，因为它是细胞内磷脂、核酸和许多其他化合物的基本成分。植物是我们食物供应的基础，全球作物生长经常受到磷供应的限制，因此，农民经常施用磷肥。植物已经进化出了应对低土壤磷有效性的机制，然而，这些机制并不一定转移到高投入依赖的作物上。了解这些机制并提高其在驯化作物物种中的效率对于开发适合我们农业系统可持续集约化的作物至关重要。植物利用生理变化从土壤中增加磷的动员和获取，如质子和酸性磷酸酶（包括具有植酸酶活性的磷酸酶）的分泌，以及为根际微生物群体提供碳的羧酸的分泌。我们将结合植物生理测定、生物化学测定、蛋白质组学和靶向代谢组学来确定在低可利用性条件下，不同种类的芸苔属植物在高效或低效吸收磷方面的关键差异。

Our motivation is to facilitate the development of crops that are more efficient in the uptake of phosphate (P), an essential macronutrient, and reduce dependency on limited P fertilizers. We intend to determine the mechanisms of protein secretion and membrane trafficking in roots that underlie differences in phosphate mobilisation in plant roots. Phosphorus is an essential macronutrient for all organisms because it is a fundamental constituent of phospholipids, nucleic acids, and numerous other compounds within cells. Plants are the basis of our food supply and globally crop growth is frequently limited by P availability, therefore, farmers frequently apply P fertilisers. Plants have evolved mechanisms to cope with low soil P availability, however, these have not necessarily been transferred to high input dependent crops. Understanding these mechanisms and improving their efficiency in domesticated crop species is critical to developing crops suitable for sustainable intensification of our agricultural systems. Plants use physiological changes to increase P mobilisation and acquisition from the soil, such as secretion of protons and acid phosphatases including those with phytase activity, and the exudation of carboxylic acids to supply root rhizosphere microbe populations with carbon. We will use a combination of plant physiological assays, in conjunction with biochemical assays and proteomics and targeted metabolomics to identify critical differences between varieties of Brassica plants that are efficient, or inefficient, in uptake of P under conditions of low availability.