TYPE 8 CAPSULE OF STAPHYLOCCUS AUREUS

描述(改编自申请人的摘要)：本提案旨在 继续李博士对金黄色葡萄球菌8型衣壳的遗传分析 多糖(CP8)。目的：1)研究cp8基因的调控。 表达对环境因素的响应；2)表征 Cap8基因的功能；以及3)确定微囊的作用 在毒力上。为了研究CP8基因的调节，PI将使用 基因融合的缺失和突变分析以确定哪些启动子 序列是基因调控所必需的。他将克隆调控基因 通过反向基因对不同的环境刺激做出反应 方法和转座子诱变。克隆的基因将被灭活 通过等位基因置换来研究这一规律。他将研究CP8基因是如何 由全球监管机构agr监管。研究CP8的功能 基因，他将使用已经产生的化学突变。每个人 通过分析累积的基因将与功能相关联 由特定基因突变产生的中间产物。重点将放在 参与链长调节、运输和聚合的基因。 为了确定微囊在发病机制中的作用，同基因缺失 突变体将在体外吞噬试验和小鼠身上进行测试。 致命性研究。

DESCRIPTION (Adapted from the applicant's abstract): This proposal is aimed at continuing Dr. Lee's genetic analysis of the S. aureus type 8 capsular polysaccharide (CP8). The aims are: 1) to study the regulation of CP8 gene expression in response to environmental factors; 2) to characterize the function of the cap8 genes; and 3) to define the role of the microcapsules in virulence. To study the regulation of CP8 genes, the PI will use deletion and mutagenesis analyses of gene fusions to define which promoter sequences are required for gene regulation. He will clone regulatory genes that respond to different environmental stimuli by a reverse genetic approach and by transposon mutagenesis. The cloned gene will be inactivated by allelic replacement to study the regulation. He will study how CP8 genes are regulated by agr, a global regulator. To study the functions of CP8 genes, he will use chemical mutants that have already been generated. Each gene will be correlated with function by analyzing the accumulated intermediate resulting from a specific gene mutation. The focus will be on genes involved in chain-length regulation, transport, and polymerization. To define the role of microcapsules in pathogenesis, isogenic deletion mutants will be tested in an in vitro phagocytic assays and by mouse lethality studies.