CORE--ISOLATED MYOCYTE AND WHOLE HEART PHYSIOLOGY

该中心的两个总体目标之一是为SCOR的项目负责人提供分离心脏细胞的设备、专业知识和技术支持，并确定分离心肌细胞的收缩功能和钙稳态。这些实验将主要在基因工程小鼠的心脏细胞上进行，并与野生型对照进行比较，尽管大鼠心脏细胞也将被使用。一些心脏细胞准备将提供给随后的生化分析（例如，酶分析，northern和Western blots），这些分析将由各自SCOR项目的研究人员进行。第二个主要是超声心动图，在后来的几年里，测量来自不同小鼠系的小鼠体内血液动力学参数。因此，Core B将支持SCOR项目负责人评估各自小鼠模型的表型，无论是在单细胞水平还是在整个动物环境中。具体目标是：1。从新生和成年小鼠和大鼠的心房和心室组织中分离心肌细胞并进行原代培养。2. 测量野生型和基因工程小鼠分离心肌细胞的收缩功能和细胞内[Ca2+]i。3. 对野生型和基因工程小鼠进行m型、二维和多普勒超声心动图研究。4. 测量野生型和基因工程小鼠的血流动力学参数（如血压、主动脉流量、心输出量、压力容积循环）。5. 为扩张型心肌病研究家族和先证者提供临床无创心功能评估。

One of the two overall objectives of this Core is to provide project leaders in the SCOR with facilities, expertise and technical support for isolating heart cells and determining contractile function and calcium homeostasis in isolated cardiomyocytes. These experiments will be done mainly on heart cells from genetically engineered mice and compared to wild type controls, although rat heart cells will also be used. Some heart cell preparations will be provide for subsequent biochemical analyses (such as, for example, enzyme assays, Norther and Western blots) which will be performed by investigators of the respective SCOR projects. The second main echocardiography and, in later years, measuring in vivo hemodynamic parameters in mice from the various mouse lines. Core B will therefore support SCOR project leaders in assessing the phenotype of their respective mouse models, both on a single cell level as well as in the whole animal context. The Specific Aims are: 1. Isolate and initiate primary cultures of cardiac myocytes from atrial and ventricular tissue of neonatal and adult mice and rats. 2. Measure contractile function and intracellular [Ca2+]i in isolated cardiomyocytes from wild type and genetically engineered mice. 3. Perform M-mode, two dimensional and Doppler echocardiography studies in wild type and genetically engineered mice. 4. Measure hemodynamic parameters (such as blood pressure, aortic flow, cardiac output, pressure volume loops) in wild type and genetically engineered mice. 5. Provide clinical non-invasive evaluation of cardiac function in study families and probands with dilated cardiomyopathy.