CORE--ANIMAL

核心--动物

• 批准号: 6411239
• 负责人: Ronald Howard Goldstein
• 金额: $ 30.86万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-01-22 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6411239
• 关键词: biomedical facility; bleomycin; disease /disorder model; genetically modified animals; histology; laboratory mouse; pulmonary fibrosis /granuloma

The Animal Core C will perform a variety of services for individual projects related to the utilization and evaluation of bleomycin-treated mice. The Specific Aims of the Animal Core include: 1. Providing a standardized model of bleomycin-treated mice to other components of the SCOR program. The lungs will be assessed histologically and biochemically for evidence of fibrosis. The Core will administer potential therapeutic agents and assess their activity in this murine model of pulmonary fibrosis. 2. Developing and/or maintaining transgenic mice strains utilized in the various components of the SCOR program. The Core will perform a variety of specialized functions on bleomycin- treated wild-type and transgenic animals for individual projects within the program. These procedures include the placement of osmotic pumps, RNA isolation and Northern blot analysis of lung tissue, in situ hybridization, and immunohistochemistry. The goal of the Core is to provide an easily accessible animal model to explore new ideas in the pathogenesis and treatment of pulmonary fibrosis.

动物核心C将为个人提供各种服务 与博来霉素治疗的使用和评价有关的项目 小鼠动物核心的具体目标包括：1。提供 博来霉素治疗的小鼠的标准化模型与其他组分的 SCOR程序。肺部将进行组织学和生化评估 寻找纤维化的证据核心将给予潜在的治疗 药物，并评估其在该肺损伤小鼠模型中的活性。 纤维化2.开发和/或维持转基因小鼠品系 用于SCOR计划的各个组成部分。 核心将对博来霉素执行各种专门功能- 为个别项目处理野生型和转基因动物， 节目这些程序包括放置渗透泵，RNA 肺组织分离和原位北方印迹分析 杂交和免疫组织化学。核心的目标是 提供了一个容易获得的动物模型，以探索新的想法， 肺纤维化的发病机制和治疗。