CORE--ANALYTICAL AND BIOMOLECULAR

对化学、生物化学和生理学的深入研究 花生四烯酸盐级联中细胞色素P450分支的意义 在以下方面取得重大进展：a)数量和重要性 生物功能归因于其代谢物，b)增加 关于代谢物结构多样性的知识，c)记录 产品生物合成的区域和立体化学决定因素 生物活性，以及d)产生、表征和 利用生物特异性探针和重组DNA方法检测 这些酶的分子和功能特征。为了支持…… 项目1-6和，以优化生产性互动和资源 利用，核心B将应用标准和既定的方法 二十烷类化合物的提取、纯化、高效液相分析、GC/MS、LS/MS、蛋白质 纯化和重组DNA操作，以：a)检测和 生物样品中二十烷类化合物的定量，b)储存，以及 合成标准的文件编制、c)储存和文件编制 免疫特异性探针，d)重组酶的部分纯化， 以及e)克隆的cDNA的扩增、纯化和记录。 将这些例行任务集中在核心B将消除 不必要且成本高昂的重复工作，并将为项目1-6提供 高效和及时地获得合成标准、生物特异性探针和 现代生物分析技术。

Intense studies of the chemistry, biochemistry, and physiological significance of the cytochrome P450 branch of the arachidonate cascade has produced significant advances in: a) the number and the importance of biological functions attributed to its metabolites, b) an increased knowledge of metabolite structural diversity, c) the documentation of regio- and stereochemical determinants of product biosynthesis and biological activity, and d) the generation, characterization, and utilization of biospecific probes and recombinant DNA methods for the molecular and functional characterization of these enzymes. In support of Projects 1-6 and, to optimize productive interactions and resources utilization, Core B will apply standard and established methods of eicosanoid extraction, purification, HPLC analysis, GC/MS, LS/MS, protein purification, and recombinant DNA manipulation, to: a) the detection and quantification of eicosanoids in biological samples, b) the storage, and documentation of synthetic standards, c) the storage, and documentation of immunospecific probes, d) the partial purification of recombinant enzymes, and e) the amplification, purification and documentation of cloned cDNAs. The centralization of these routine tasks in Core B will eliminate unnecessary and costly duplications and will provide projects 1-6 with efficient and timely access to synthetic standards, biospecific probes and modern bioanalytical techniques.