CORE--IMMUNOELECTRON MICROSCOPY

核心--免疫电子显微镜

• 批准号: 6448178
• 负责人: MARILYN Gist FARQUHAR
• 金额: $ 15.83万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-20 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6448178
• 关键词: bioimaging /biomedical imaging; biomedical facility; cryostat; fluoresceins; immunocytochemistry; immunoelectron microscopy; immunofluorescence technique; immunoperoxidase; protein localization; sectioning

Immunocytochemistry has been a powerful tool in research in cell biology. It allows the precise localization of individual proteins to cell organelles and--when carried to the electron microscope level--even to specific subcompartments thereof. It provides information on localization of proteins in intact cells and therefore is essential in that it complements cells fractionation and biochemical approaches. To apply immunocytochemical methods at the electron microscope level is technically demanding and requires extensive instrumentation and highly skilled and experienced personnel. The purpose of Core B is to provide this expertise and make it possible for all member of the Program Project to apply these techniques in their program-related research. Personnel associated with the Core have considerable experience in all major approaches current used in immunocytochemistry involving immunofluorescence, immunoperoxidase techniques, and immunogold labeling of ultrathin cryosections. The Core Director has used these techniques in her research over 15 years, and Mr. McCaffery, the Core's Technical Director, has had over 15 years experiences in this area and has co-authored a number of publications, including 10 during the current funding period using these techniques. During the last four years under the auspices of the Program Project, we have operated the facility as a state-of-the-ar Core to serve program members. The Core has been heavily used by all Project leaders as attested to by the fact that 25 publications have utilized core facilities and/or personnel. Availability of the expertise of the expertise of the core has provided new information that led to new research direction for the Project Leaders.

免疫细胞化学已成为细胞生物学研究的有力工具。 它允许单个蛋白质精确定位到细胞 细胞器和-当进行到电子显微镜水平-甚至 其特定的子分区。它提供有关本地化的信息 蛋白质在完整的细胞中，因此是必不可少的， 补充细胞分级分离和生物化学方法。申请 免疫细胞化学方法在电子显微镜水平上， 要求高，需要大量的仪器和高度熟练的， 经验丰富的人员。核心B的目的是提供这种专业知识 并使计划项目的所有成员都能应用这些 技术在他们的程序相关的研究。相关人员 核心小组在目前使用所有主要方法方面都有相当丰富的经验 在涉及免疫荧光、免疫过氧化物酶 技术和免疫金标记的冷冻切片。核心 在过去的15年里，导演在她的研究中使用了这些技术。 McCaffery，核心的技术总监，已经有超过15年的时间， 在这一领域的经验，并共同撰写了一些出版物， 包括本供资期间使用这些技术的10个项目。 在过去的四年里，在方案项目的主持下，我们 已将该设施作为国家级核心服务计划运营 成员核心已经被所有项目领导人大量使用， 25份出版物利用了核心设施和/或 人员的核心专家的专门知识的可用性 提供了新的信息，导致新的研究方向， 项目负责人。